MicroRNA-132 Interact with p250GAPCdc42 Pathway in the Hippocampal Neuronal Culture Model of Acquired Epilepsy and Associated with Epileptogenesis Process
Joint Authors
Yuan, Jinxian
Huang, Hao
Zhou, Xin
Liu, Xi
Ou, Shu
Xu, Tao
Li, Ruohan
Ma, Limin
Chen, Yangmei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-08
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Increasing evidence suggests that epilepsy is the result of synaptic reorganization and pathological excitatory loop formation in the central nervous system; however, the mechanisms that regulate this process are not well understood.
We proposed that microRNA-132 (miR-132) and p250GAP might play important roles in this process by activating the downstream Rho GTPase family.
We tested this hypothesis using a magnesium-free medium-induced epileptic model of cultured hippocampal neurons.
We investigated whether miR-132 regulates GTPase activity through p250GAP and found that Cdc42 was significantly activated in our experimental model.
Silencing miR-132 inhibited the electrical excitability level of cultured epileptic neurons, whereas silencing p250GAP had an opposite effect.
In addition, we verified the effect of miR-132 in vivo and found that silencing miR-132 inhibited the aberrant formation of dendritic spines and chronic spontaneous seizure in a lithium-pilocarpine-induced epileptic mouse model.
Finally, we confirmed that silencing miR-132 has a neuroprotective effect on cultured epileptic neurons; however, this effect did not occur through the p250GAP pathway.
Generally, silencing miR-132 may suppress spontaneous seizure activity through the miR-132/p250GAP/Cdc42 pathway by regulating the morphology and electrophysiology of dendritic spines; therefore, miR-132 may serve as a potential target for the development of antiepileptic drugs.
American Psychological Association (APA)
Yuan, Jinxian& Huang, Hao& Zhou, Xin& Liu, Xi& Ou, Shu& Xu, Tao…[et al.]. 2016. MicroRNA-132 Interact with p250GAPCdc42 Pathway in the Hippocampal Neuronal Culture Model of Acquired Epilepsy and Associated with Epileptogenesis Process. Neural Plasticity،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1113177
Modern Language Association (MLA)
Yuan, Jinxian…[et al.]. MicroRNA-132 Interact with p250GAPCdc42 Pathway in the Hippocampal Neuronal Culture Model of Acquired Epilepsy and Associated with Epileptogenesis Process. Neural Plasticity No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1113177
American Medical Association (AMA)
Yuan, Jinxian& Huang, Hao& Zhou, Xin& Liu, Xi& Ou, Shu& Xu, Tao…[et al.]. MicroRNA-132 Interact with p250GAPCdc42 Pathway in the Hippocampal Neuronal Culture Model of Acquired Epilepsy and Associated with Epileptogenesis Process. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1113177
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113177