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Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo
Joint Authors
Shu, Yilai
Tao, Yong
Li, Wenyan
Shen, Jun
Wang, Zhengmin
Chen, Zheng-Yi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Mammalian inner ear harbors diverse cell types that are essential for hearing and balance.
Adenovirus is one of the major vectors to deliver genes into the inner ear for functional studies and hair cell regeneration.
To identify adenovirus vectors that target specific cell subtypes in the inner ear, we studied three adenovirus vectors, carrying a reporter gene encoding green fluorescent protein (GFP) from two vendors or with a genome editing gene Cre recombinase (Cre), by injection into postnatal days 0 (P0) and 4 (P4) mouse cochlea through scala media by cochleostomy in vivo.
We found three adenovirus vectors transduced mouse inner ear cells with different specificities and expression levels, depending on the type of adenoviral vectors and the age of mice.
The most frequently targeted region was the cochlear sensory epithelium, including auditory hair cells and supporting cells.
Adenovirus with GFP transduced utricular supporting cells as well.
This study shows that adenovirus vectors are capable of efficiently and specifically transducing different cell types in the mammalian inner ear and provides useful tools to study inner ear gene function and to evaluate gene therapy to treat hearing loss and vestibular dysfunction.
American Psychological Association (APA)
Shu, Yilai& Tao, Yong& Li, Wenyan& Shen, Jun& Wang, Zhengmin& Chen, Zheng-Yi. 2016. Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo. Neural Plasticity،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1113384
Modern Language Association (MLA)
Shu, Yilai…[et al.]. Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo. Neural Plasticity No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1113384
American Medical Association (AMA)
Shu, Yilai& Tao, Yong& Li, Wenyan& Shen, Jun& Wang, Zhengmin& Chen, Zheng-Yi. Adenovirus Vectors Target Several Cell Subtypes of Mammalian Inner Ear In Vivo. Neural Plasticity. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1113384
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113384