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Response of Mouse Zygotes Treated with Mild Hydrogen Peroxide as a Model to Reveal Novel Mechanisms of Oxidative Stress-Induced Injury in Early Embryos
Joint Authors
Qian, Diting
Li, Zhiling
Zhang, Yuting
Huang, Yue
Wu, Que
Ru, Gaizhen
Chen, Man
Wang, Bin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Our study aimed to develop embryo models to evaluate the impact of oxidative stress on embryo development.
Mouse zygotes, which stayed at G1 phase, were treated with prepared culture medium (containing 0.00, 0.01, 0.02, 0.03, 0.04, 0.05, or 0.1 mM hydrogen peroxide (H2O2)) for 30 min in experiment 1.
The dose-effects of H2O2 on embryo development were investigated via comparisons of the formation rate at each stage (2- and 4-cell embryos and blastocysts).
Experiment 2 was carried out to compare behaviors of embryos in a mild oxidative-stressed status (0.03 mM H2O2) with those in a control (0 mM H2O2).
Reactive oxygen species (ROS) levels, variation of mitochondrial membrane potential (MMP), expression of γH2AX, and cell apoptosis rate of blastocyst were detected.
We observed a dose-dependent decrease on cleavage and blastocyst rates.
Besides, higher level of ROS, rapid reduction of MMP, and the appearance of γH2AX revealed that embryos are injured early in mild oxidative stress.
Additionally, γH2AX may involve during DNA damage response in early embryos.
And the apoptotic rate of blastocyst may significantly increase when DNA damage repair is inadequate.
Most importantly, our research provides embryo models to study cell cycle regulation and DNA damage response under condition of different levels of oxidative stress.
American Psychological Association (APA)
Qian, Diting& Li, Zhiling& Zhang, Yuting& Huang, Yue& Wu, Que& Ru, Gaizhen…[et al.]. 2016. Response of Mouse Zygotes Treated with Mild Hydrogen Peroxide as a Model to Reveal Novel Mechanisms of Oxidative Stress-Induced Injury in Early Embryos. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113620
Modern Language Association (MLA)
Qian, Diting…[et al.]. Response of Mouse Zygotes Treated with Mild Hydrogen Peroxide as a Model to Reveal Novel Mechanisms of Oxidative Stress-Induced Injury in Early Embryos. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113620
American Medical Association (AMA)
Qian, Diting& Li, Zhiling& Zhang, Yuting& Huang, Yue& Wu, Que& Ru, Gaizhen…[et al.]. Response of Mouse Zygotes Treated with Mild Hydrogen Peroxide as a Model to Reveal Novel Mechanisms of Oxidative Stress-Induced Injury in Early Embryos. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113620
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113620