Differential Regulation of the Extracellular CysteineCystine Redox State (EhCySS) by Lung Fibroblasts from Young and Old Mice
Joint Authors
Watson, Walter H.
Burke, Tom J.
Zelko, Igor N.
Torres-González, Edilson
Ritzenthaler, Jeffrey D.
Roman, Jesse
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-25
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Aging is associated with progressive oxidation of plasma cysteine (Cys)/cystine (CySS) redox state, expressed as E h C y S S .
Cultured cells condition their media to reproduce physiological E h C y S S , but it is unknown whether aged cells produce a more oxidized extracellular environment reflective of that seen in vivo.
In the current study, we isolated primary lung fibroblasts from young and old female mice and measured the media E h C y S S before and after challenge with Cys or CySS.
We also measured expression of genes related to redox regulation and fibroblast function.
These studies revealed that old fibroblasts produced a more oxidizing extracellular E h C y S S than young fibroblasts and that old fibroblasts had a decreased capacity to recover from an oxidative challenge due to a slower rate of reduction of CySS to Cys.
These defects were associated with 10-fold lower expression of the Slc7a11 subunit of the xCT cystine-glutamate transporter.
Extracellular superoxide dismutase (Sod3) was the only antioxidant or thiol-disulfide regulating enzyme among 36 examined that was downregulated in old fibroblasts by more than 2-fold, but there were numerous changes in extracellular matrix components.
Thus, aging fibroblasts not only contribute to remodeling of the extracellular matrix but also have a profound effect on the extracellular redox environment.
American Psychological Association (APA)
Watson, Walter H.& Burke, Tom J.& Zelko, Igor N.& Torres-González, Edilson& Ritzenthaler, Jeffrey D.& Roman, Jesse. 2016. Differential Regulation of the Extracellular CysteineCystine Redox State (EhCySS) by Lung Fibroblasts from Young and Old Mice. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113628
Modern Language Association (MLA)
Watson, Walter H.…[et al.]. Differential Regulation of the Extracellular CysteineCystine Redox State (EhCySS) by Lung Fibroblasts from Young and Old Mice. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113628
American Medical Association (AMA)
Watson, Walter H.& Burke, Tom J.& Zelko, Igor N.& Torres-González, Edilson& Ritzenthaler, Jeffrey D.& Roman, Jesse. Differential Regulation of the Extracellular CysteineCystine Redox State (EhCySS) by Lung Fibroblasts from Young and Old Mice. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113628
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113628