Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line
Joint Authors
Maegawa, Hiroshi
Yamamoto, Hirotaka
Morino, Katsutaro
Mengistu, Lemecha
Ishibashi, Taishi
Kiriyama, Kohei
Ikami, Takao
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-02
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Amla is one of the most important plants in Indian traditional medicine and has been shown to improve various age-related disorders while decreasing oxidative stress.
Mitochondrial dysfunction is a proposed cause of aging through elevated oxidative stress.
In this study, we investigated the effects of Amla on mitochondrial function in C2C12 myotubes, a murine skeletal muscle cell model with abundant mitochondria.
Based on cell flux analysis, treatment with an extract of Amla fruit enhanced mitochondrial spare respiratory capacity, which enables cells to overcome various stresses.
To further explore the mechanisms underlying these effects on mitochondrial function, we analyzed mitochondrial biogenesis and antioxidant systems, both proposed regulators of mitochondrial spare respiratory capacity.
We found that Amla treatment stimulated both systems accompanied by AMPK and Nrf2 activation.
Furthermore, we found that Amla treatment exhibited cytoprotective effects and lowered reactive oxygen species (ROS) levels in cells subjected to t-BHP-induced oxidative stress.
These effects were accompanied by increased oxygen consumption, suggesting that Amla protected cells against oxidative stress by using enhanced spare respiratory capacity to produce more energy.
Thus we identified protective effects of Amla, involving activation of mitochondrial function, which potentially explain its various effects on age-related disorders.
American Psychological Association (APA)
Yamamoto, Hirotaka& Morino, Katsutaro& Mengistu, Lemecha& Ishibashi, Taishi& Kiriyama, Kohei& Ikami, Takao…[et al.]. 2016. Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113642
Modern Language Association (MLA)
Yamamoto, Hirotaka…[et al.]. Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113642
American Medical Association (AMA)
Yamamoto, Hirotaka& Morino, Katsutaro& Mengistu, Lemecha& Ishibashi, Taishi& Kiriyama, Kohei& Ikami, Takao…[et al.]. Amla Enhances Mitochondrial Spare Respiratory Capacity by Increasing Mitochondrial Biogenesis and Antioxidant Systems in a Murine Skeletal Muscle Cell Line. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113642
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113642