t-BHQ Provides Protection against Lead Neurotoxicity via Nrf2HO-1 Pathway
Joint Authors
Ye, Fang
Li, Xiaoyi
Li, Lili
Yuan, Jing
Chen, Jun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-21
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
The neurotoxicity of lead has been well established, and oxidative stress is strongly associated with lead-induced neurotoxicity.
Nrf2 is important for protection against oxidative stress in many disease models.
We applied t-BHQ, which is an Nrf2 activator, to investigate the possible role of Nrf2 in the protection against lead neurotoxicity.
t-BHQ significantly attenuated the oxidative stress in developmental rats by decreasing MDA level, as well as by increasing SOD activity and GSH content, in the hippocampus and frontal cortex.
Furthermore, neuronal apoptosis was detected by Nissl staining, and Bax expression was inhibited in the t-BHQ-treated group.
Results showed that t-BHQ suppressed ROS production and caspase 3/7 activity but increased intracellular GSH content, in SH-SY5Y cells under lead exposure.
Moreover, in vivo and in vitro, t-BHQ enhanced the nuclear translocation of Nrf2 and binding to ARE areas but did not induce Nrf2 transcription.
These phenomena were confirmed using RT-PCR, EMSA, Western blot, and immunofluorescence analyses.
Subsequent upregulation of the expression of HO-1, NQO1, and GCLC was observed.
However, knockdown of Nrf2 or HO-1 adversely affected the protective effects of t-BHQ against lead toxicity in SH-SY5Y cells.
Thus, t-BHQ can protect against lead neurotoxicity, depending on the Nrf2/HO-1 pathway.
American Psychological Association (APA)
Ye, Fang& Li, Xiaoyi& Li, Lili& Yuan, Jing& Chen, Jun. 2015. t-BHQ Provides Protection against Lead Neurotoxicity via Nrf2HO-1 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1113680
Modern Language Association (MLA)
Ye, Fang…[et al.]. t-BHQ Provides Protection against Lead Neurotoxicity via Nrf2HO-1 Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1113680
American Medical Association (AMA)
Ye, Fang& Li, Xiaoyi& Li, Lili& Yuan, Jing& Chen, Jun. t-BHQ Provides Protection against Lead Neurotoxicity via Nrf2HO-1 Pathway. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1113680
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113680