Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult BalbC Mice during REM Sleep Deprivation
Joint Authors
González-Pérez, Oscar
Gonzalez-Castaneda, Rocio E.
Flores-Soto, Mario Eduardo
Chaparro-Huerta, Verónica
Jave-Suarez, Luis Felipe
Soto-Rodríguez, Sofía
Rusanova, Iryna
López-Armas, Gabriela
Acuna-Castroviejo, Dario
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-07
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Purpose.
The aim of this study was to assess the effect of melatonin in the proliferation of neural progenitors, melatonin concentration, and antiapoptotic proteins in the hippocampus of adult mice exposed to 96 h REM sleep deprivation (REMSD) prophylactic administration of melatonin for 14 days.
Material and Methods.
Five groups of Balb/C mice were used: (1) control, (2) REMSD, (3) melatonin (10 mg/kg) plus REMSD, (4) melatonin and intraperitoneal luzindole (once a day at 5 mg/kg) plus REMSD, and (5) luzindole plus REMSD.
To measure melatonin content in hippocampal tissue we used HPLC.
Bcl-2 and Bcl-xL proteins were measured by Western Blot and neurogenesis was determined by injecting 5-bromo-2-deoxyuridine (BrdU) and BrdU/nestin expressing cells in the subgranular zone of the dentate gyrus were quantified by epifluorescence.
Results.
The melatonin-treated REMSD group showed an increased neural precursor in 44% with respect to the REMSD group and in 28% when contrasted with the control group ( P < 0.021 ).
The melatonin-treated REMSD group also showed the highest expression of Bcl-2 and Bcl-xL as compared to the rest of the groups.
Conclusion.
The exogenous administration of melatonin restores the tissue levels of sleep-deprived group and appears to be an efficient neuroprotective agent against the deleterious effects of REMSD.
American Psychological Association (APA)
López-Armas, Gabriela& Flores-Soto, Mario Eduardo& Chaparro-Huerta, Verónica& Jave-Suarez, Luis Felipe& Soto-Rodríguez, Sofía& Rusanova, Iryna…[et al.]. 2016. Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult BalbC Mice during REM Sleep Deprivation. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113685
Modern Language Association (MLA)
López-Armas, Gabriela…[et al.]. Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult BalbC Mice during REM Sleep Deprivation. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1113685
American Medical Association (AMA)
López-Armas, Gabriela& Flores-Soto, Mario Eduardo& Chaparro-Huerta, Verónica& Jave-Suarez, Luis Felipe& Soto-Rodríguez, Sofía& Rusanova, Iryna…[et al.]. Prophylactic Role of Oral Melatonin Administration on Neurogenesis in Adult BalbC Mice during REM Sleep Deprivation. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113685
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113685