BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats
Joint Authors
Goraca, Anna
Zebrowska, Marta
Kowalczyk, Agata
Jeleń, Agnieszka
Balcerczak, Ewa
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-28
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Little is understood of skeletal muscle tissue in terms of oxidative stress and inflammation.
Endothelin-1 is an endogenous, vasoconstrictive peptide which can induce overproduction of reactive oxygen species and proinflammatory cytokines.
The aim of this study was to evaluate whether BQ123, an endothelin-A receptor antagonist, influences the level of TNF-α, IL-6, SOD-1, HO-1, Nrf2 mRNA, and NF-κB subunit RelA/p65 mRNA in the femoral muscle obtained from endotoxemic rats.
Male Wistar rats were divided into 4 groups (n=6) and received iv (1) saline (control), (2) LPS (15 mg/kg), (3) BQ123 (1 mg/kg), (4) BQ123 (1 mg/kg), and LPS (15 mg/kg, resp.) 30 min later.
Injection of LPS led to significant increase in levels of RelA/p65 mRNA, TNF-α, and IL-6, while content of SOD-1, HO-1, and Nrf2 mRNA was unchanged.
Administration of BQ123 prior to LPS challenge resulted in a significant reduction in RelA/p65 mRNA, TNF-α, and IL-6 levels, as well as markedly elevated concentrations of SOD-1, HO-1, and Nrf2 mRNA.
BQ123 appears to enhance antioxidant defense and prevent production of TNF-α and IL-6 in skeletal muscle of LPS-treated rat.
In conclusion, endothelin-A receptor antagonism exerts significant impact on the skeletal muscle favouring anti-inflammatory effects and protection against oxidative stress.
American Psychological Association (APA)
Kowalczyk, Agata& Jeleń, Agnieszka& Zebrowska, Marta& Balcerczak, Ewa& Goraca, Anna. 2015. BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1113696
Modern Language Association (MLA)
Kowalczyk, Agata…[et al.]. BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1113696
American Medical Association (AMA)
Kowalczyk, Agata& Jeleń, Agnieszka& Zebrowska, Marta& Balcerczak, Ewa& Goraca, Anna. BQ123 Stimulates Skeletal Muscle Antioxidant Defense via Nrf2 Activation in LPS-Treated Rats. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1113696
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113696