Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity
Joint Authors
Hasnaoui, Mounia
Balogh, Balázs
Matyus, Peter
Fernández-Quintela, Alfredo
Rodríguez, Víctor
Mercader, Josep
Portillo, Maria P.
Carpéné, Christian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Resveratrol has been reported to inhibit monoamine oxidases (MAO).
Many substrates or inhibitors of neuronal MAO interact also with other amine oxidases (AO) in peripheral organs, such as semicarbazide-sensitive AO (SSAO), known as primary amine oxidase, absent in neurones, but abundant in adipocytes.
We asked whether phenolic compounds (resveratrol, pterostilbene, quercetin, and caffeic acid) behave as MAO and SSAO inhibitors.
AO activity was determined in human adipose tissue.
Computational docking and glucose uptake assays were performed in 3D models of human AO proteins and in adipocytes, respectively.
Phenolic compounds fully inhibited the fluorescent detection of H2O2 generated during MAO and SSAO activation by tyramine and benzylamine.
They also quenched H2O2-induced fluorescence in absence of biological material and were unable to abolish the oxidation of radiolabelled tyramine and benzylamine.
Thus, phenolic compounds hampered H2O2 detection but did not block AO activity.
Only resveratrol and quercetin partially impaired MAO-dependent [14C]-tyramine oxidation and behaved as MAO inhibitors.
Phenolic compounds counteracted the H2O2-dependent benzylamine-stimulated glucose transport.
This indicates that various phenolic compounds block downstream effects of H2O2 produced by biogenic or exogenous amine oxidation without directly inhibiting AO.
Phenolic compounds remain of interest regarding their capacity to limit oxidative stress rather than inhibiting AO.
American Psychological Association (APA)
Carpéné, Christian& Hasnaoui, Mounia& Balogh, Balázs& Matyus, Peter& Fernández-Quintela, Alfredo& Rodríguez, Víctor…[et al.]. 2016. Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1113705
Modern Language Association (MLA)
Carpéné, Christian…[et al.]. Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1113705
American Medical Association (AMA)
Carpéné, Christian& Hasnaoui, Mounia& Balogh, Balázs& Matyus, Peter& Fernández-Quintela, Alfredo& Rodríguez, Víctor…[et al.]. Dietary Phenolic Compounds Interfere with the Fate of Hydrogen Peroxide in Human Adipose Tissue but Do Not Directly Inhibit Primary Amine Oxidase Activity. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1113705
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113705