Hydrogen Sulfide Regulates the [Ca2+]i Level in the Primary Medullary Neurons
Joint Authors
Wang, Rui
Liu, Xiaoni
Zhang, Nana
Lu, Ning
Ding, Yingjiong
Cao, Dongqing
Huang, Ying
Chen, Xiangjun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the present study, we attempted to elucidate mechanisms for the regulation of intracellular calcium levels by H2S in primary rat medullary neurons.
Our results showed that NaHS significantly increased the level of Ca2+i in rat medullary neurons in a concentration-dependent manner.
L-Cysteine and SAM significantly raised the level of Ca2+i in the medullary neurons while HA and/or AOAA produced a reversal effect.
In addition, L-cysteine and SAM significantly increased but HA and/or AOAA decreased the production of H2S in the cultured neurons.
The Ca2+i elevation induced by H2S was significantly diminished by EGTA-Ca2+-free solutions, and this elevation was also reduced by nifedipine or nimodipine and mibefradil, suggesting the role of L-type and/or T-type Ca2+ channels.
Moreover, the effect of H2S on Ca2+i level in neurons was significantly attenuated by BAPTA-AM and thapsigargin, suggesting the source of Ca2+.
Therefore, we concluded that both exogenous and endogenous H2S elevates Ca2+i level in primarily cultured rat medullary neurons via both increasing calcium influx and mobilizing intracellular Ca2+ stores from ER.
American Psychological Association (APA)
Liu, Xiaoni& Zhang, Nana& Ding, Yingjiong& Cao, Dongqing& Huang, Ying& Chen, Xiangjun…[et al.]. 2016. Hydrogen Sulfide Regulates the [Ca2+]i Level in the Primary Medullary Neurons. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113738
Modern Language Association (MLA)
Liu, Xiaoni…[et al.]. Hydrogen Sulfide Regulates the [Ca2+]i Level in the Primary Medullary Neurons. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1113738
American Medical Association (AMA)
Liu, Xiaoni& Zhang, Nana& Ding, Yingjiong& Cao, Dongqing& Huang, Ying& Chen, Xiangjun…[et al.]. Hydrogen Sulfide Regulates the [Ca2+]i Level in the Primary Medullary Neurons. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113738
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113738