Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1α Expression in HT22 Hippocampal Neurons
Joint Authors
Yue, Liang
Zhao, Lei
Liu, Haixiao
Li, Xia
Wang, Bodong
Guo, Hao
Gao, Li
Feng, Dayun
Qu, Yan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-30
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Glutamate- (Glu-) induced excitotoxicity plays a critical role in stroke.
This study aimed to investigate the effects of APN on Glu-induced injury in HT22 neurons.
HT22 neurons were treated with Glu in the absence or the presence of an APN peptide.
Cell viability was assessed using the MTT assay, while cell apoptosis was evaluated using TUNEL staining.
Levels of LDH, MDA, SOD, and GSH-Px were detected using the respective kits, and ROS levels were detected using dichlorofluorescein diacetate.
Western blot was used to detect the expression levels of silent information regulator 1 (SIRT1), peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α), cleaved caspase-3, Bax, and Bcl-2.
In addition to the western blot, immunofluorescence was used to investigate the expression levels of SIRT1 and PGC-1α.
Our results suggest that APN peptide increased cell viability, SOD, and GSH-Px levels and decreased LDH release, ROS and MDA levels, and cell apoptosis.
APN peptide upregulated the expression of SIRT1, PGC-1α, and Bcl-2 and downregulated the expression of cleaved caspase-3 and Bax.
Furthermore, the protective effects of the APN peptide were abolished by SIRT1 siRNA.
Our findings suggest that APN peptide protects HT22 neurons against Glu-induced injury by inhibiting neuronal apoptosis and activating SIRT1-dependent PGC-1α signaling.
American Psychological Association (APA)
Yue, Liang& Zhao, Lei& Liu, Haixiao& Li, Xia& Wang, Bodong& Guo, Hao…[et al.]. 2016. Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1α Expression in HT22 Hippocampal Neurons. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113758
Modern Language Association (MLA)
Yue, Liang…[et al.]. Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1α Expression in HT22 Hippocampal Neurons. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1113758
American Medical Association (AMA)
Yue, Liang& Zhao, Lei& Liu, Haixiao& Li, Xia& Wang, Bodong& Guo, Hao…[et al.]. Adiponectin Protects against Glutamate-Induced Excitotoxicity via Activating SIRT1-Dependent PGC-1α Expression in HT22 Hippocampal Neurons. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113758
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113758