![](/images/graphics-bg.png)
Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells
Joint Authors
Salvetti, Marco
Falabella, Micol
Magnifico, Maria Chiara
Santini, Paolo
Radić, Kristina
Chessa, Luciana
Coarelli, Giulia
Buscarinu, Maria Chiara
Mechelli, Rosella
Giuffrè, Alessandro
Sarti, Paolo
Forte, Elena
Arese, Marzia
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Here we have collected evidence suggesting that chronic changes in the NO homeostasis and the rise of reactive oxygen species bioavailability can contribute to cell dysfunction in Leber’s hereditary optic neuropathy (LHON) patients.
We report that peripheral blood mononuclear cells (PBMCs), derived from a female LHON patient with bilateral reduced vision and carrying the pathogenic mutation 11778/ND4, display increased levels of reactive oxygen species (ROS) and reactive nitrogen species (RNS), as revealed by flow cytometry, fluorometric measurements of nitrite/nitrate, and 3-nitrotyrosine immunodetection.
Moreover, viability assays with the tetrazolium dye MTT showed that lymphoblasts from the same patient are more sensitive to prolonged NO exposure, leading to cell death.
Taken together these findings suggest that oxidative and nitrosative stress cooperatively play an important role in driving LHON pathology when excess NO remains available over time in the cell environment.
American Psychological Association (APA)
Magnifico, Maria Chiara& Santini, Paolo& Arese, Marzia& Giuffrè, Alessandro& Radić, Kristina& Chessa, Luciana…[et al.]. 2015. Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113782
Modern Language Association (MLA)
Magnifico, Maria Chiara…[et al.]. Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1113782
American Medical Association (AMA)
Magnifico, Maria Chiara& Santini, Paolo& Arese, Marzia& Giuffrè, Alessandro& Radić, Kristina& Chessa, Luciana…[et al.]. Evidence for Detrimental Cross Interactions between Reactive Oxygen and Nitrogen Species in Leber’s Hereditary Optic Neuropathy Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113782
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113782