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Wild Raspberry Subjected to Simulated Gastrointestinal Digestion Improves the Protective Capacity against Ethyl Carbamate-Induced Oxidative Damage in Caco-2 Cells
Joint Authors
Chen, Wei
Xu, Yang
Zhang, Lingxia
Li, Ya
Zheng, Xiaodong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-16
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Ethyl carbamate (EC), a probable human carcinogen, occurs widely in many fermented foods.
Previous studies indicated that EC-induced cytotoxicity was associated with oxidative stress.
Wild raspberries are rich in polyphenolic compounds, which possess potent antioxidant activity.
This study was conducted to investigate the protective effect of wild raspberry extracts produced before (RE) and after in vitro simulated gastrointestinal digestion (RD) on EC-induced oxidative damage in Caco-2 cells.
Our primary data showed that ethyl carbamate could result in cytotoxicity and genotoxicity in Caco-2 cells and raspberry extract after digestion (RD) may be more effective than that before digestion (RE) in attenuating toxicity caused by ethyl carbamate.
Further investigation by fluorescence microscope revealed that RD may significantly ameliorate EC-induced oxidative damage by scavenging the overproduction of intracellular reactive oxygen species (ROS), maintaining mitochondrial function and preventing glutathione (GSH) depletion.
In addition, HPLC-ESI-MS results showed that the contents of identified polyphenolic compounds (esculin, kaempferol O-hexoside, and pelargonidin O-hexoside) were remarkably increased after digestion, which might be related to the better protective effect of RD.
Overall, our results demonstrated that raspberry extract undergoing simulated gastrointestinal digestion may improve the protective effect against EC-induced oxidative damage in Caco-2 cells.
American Psychological Association (APA)
Chen, Wei& Xu, Yang& Zhang, Lingxia& Li, Ya& Zheng, Xiaodong. 2015. Wild Raspberry Subjected to Simulated Gastrointestinal Digestion Improves the Protective Capacity against Ethyl Carbamate-Induced Oxidative Damage in Caco-2 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113803
Modern Language Association (MLA)
Chen, Wei…[et al.]. Wild Raspberry Subjected to Simulated Gastrointestinal Digestion Improves the Protective Capacity against Ethyl Carbamate-Induced Oxidative Damage in Caco-2 Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1113803
American Medical Association (AMA)
Chen, Wei& Xu, Yang& Zhang, Lingxia& Li, Ya& Zheng, Xiaodong. Wild Raspberry Subjected to Simulated Gastrointestinal Digestion Improves the Protective Capacity against Ethyl Carbamate-Induced Oxidative Damage in Caco-2 Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113803
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113803