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Protective Effect of Peroxisome Proliferator-Activated Receptor α Activation against Cardiac Ischemia-Reperfusion Injury Is Related to Upregulation of Uncoupling Protein-3
Joint Authors
Song, Jong Wook
Kim, Hyo Jung
Lee, Hyelin
Kim, Jae-woo
Kwak, Young-Lan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Activation of peroxisome proliferator-activated receptor α (PPARα) confers cardioprotection, while its mechanism remains elusive.
We investigated the protective effect of PPARα activation against cardiac ischemia-reperfusion injury in terms of the expression of uncoupling protein (UCP).
Myocardial infarct size and UCP expression were measured in rats treated with WY-14643 20 mg/kg, a PPARα ligand, or vehicle.
WY-14643 increased UCP3 expression in vivo.
Myocardial infarct size was decreased in the WY-14643 group (76 ± 8% versus 42 ± 12%, P<0.05).
During reperfusion, the incidence of arrhythmia was higher in the control group compared with the WY-14643 group (9/10 versus 3/10, P<0.05).
H9c2 cells were incubated for 24 h with WY-14643 or vehicle.
WY-14643 increased UCP3 expression in H9c2 cells.
WY-14643 decreased hypoxia-stimulated ROS production.
Cells treated with WY-14643 were more resistant to hypoxia-reoxygenation than the untreated cells.
Knocking-down UCP3 by siRNA prevented WY-14643 from attenuating the production of ROS.
UCP3 siRNA abolished the effect of WY-14643 on cell viability against hypoxia-reoxygenation.
In summary, administration of PPARα agonist WY-14643 mitigated the extent of myocardial infarction and incidence of reperfusion-induced arrhythmia.
PPARα activation conferred cytoprotective effect against hypoxia-reoxygenation.
Associated mechanisms involved increased UCP3 expression and resultant attenuation of ROS production.
American Psychological Association (APA)
Song, Jong Wook& Kim, Hyo Jung& Lee, Hyelin& Kim, Jae-woo& Kwak, Young-Lan. 2015. Protective Effect of Peroxisome Proliferator-Activated Receptor α Activation against Cardiac Ischemia-Reperfusion Injury Is Related to Upregulation of Uncoupling Protein-3. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113828
Modern Language Association (MLA)
Song, Jong Wook…[et al.]. Protective Effect of Peroxisome Proliferator-Activated Receptor α Activation against Cardiac Ischemia-Reperfusion Injury Is Related to Upregulation of Uncoupling Protein-3. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113828
American Medical Association (AMA)
Song, Jong Wook& Kim, Hyo Jung& Lee, Hyelin& Kim, Jae-woo& Kwak, Young-Lan. Protective Effect of Peroxisome Proliferator-Activated Receptor α Activation against Cardiac Ischemia-Reperfusion Injury Is Related to Upregulation of Uncoupling Protein-3. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113828
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113828