The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species
Joint Authors
Shen, Xin
Li, Xuqi
Lei, Jianjun
Ma, Qingyong
Zhang, Lun
Li, Jiahui
Chen, Xin
Chen, Ke
Li, Wei
Shan, Tao
Ma, Jiguang
Jiang, Zhengdong
Zong, Liang
Yu, Shuo
Duan, Wanxing
Xu, Qinhong
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-22
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Nrf2 (NF-E2-related factor 2) pathway and autophagy both can respond to oxidative stress to promote cancer cells to survive in the tumor microenvironment.
We, therefore, explored the relevance between Nrf2 pathway and autophagy in pancreatic cancer cells upon stimulation of reactive oxygen species (ROS).
Pancreatic cancer cells were cultured under controlled ROS stressing condition or basal condition.
Different inhibitors were used to prevent autophagy at particular stages.
Nrf2 siRNA was used to inhibit Nrf2 pathway activation.
Ad-mRFP-GFP-LC3 infection was used to monitor autophagic flux.
The result shows that a small amount of exogenous hydrogen peroxide (H2O2) can significantly improve the level of intracellular ROS.
Moreover, our findings indicate that ROS promotes the activation of both Nrf2 pathway and autophagy in pancreatic cancer cells.
Moreover, our data demonstrate that suppression of autophagic activity at particular stages results in an increased promotion of Nrf2 pathway activation upon ROS stimulation.
Furthermore, we found that silencing of Nrf2 promotes autophagy upon ROS stimulation.
In addition, Nrf2 interference effectively promotes autophagic flux upon ROS stimulation.
In summary, our findings suggest that Nrf2 pathway and autophagy have a negative interaction with each other upon ROS stimulation.
American Psychological Association (APA)
Zhang, Lun& Li, Jiahui& Ma, Jiguang& Chen, Xin& Chen, Ke& Jiang, Zhengdong…[et al.]. 2015. The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113865
Modern Language Association (MLA)
Zhang, Lun…[et al.]. The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1113865
American Medical Association (AMA)
Zhang, Lun& Li, Jiahui& Ma, Jiguang& Chen, Xin& Chen, Ke& Jiang, Zhengdong…[et al.]. The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1113865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113865