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High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster
Joint Authors
Franco, Jeferson Luis
Zemolin, A. P. P.
Prigol, Marina
Trindade de Paula, Mariane
Poetini Silva, Márcia Rósula
Machado Araujo, Stífani
Cardoso Bortolotto, Vandreza
Barreto Meichtry, Luana
Wallau, Gabriel L.
Jesse, Cristiano Ricardo
Posser, Thaís
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The consumption of a high-fat diet (HFD) causes alteration in normal metabolism affecting lifespan of flies; however molecular mechanism associated with this damage in flies is not well known.
This study evaluates the effects of ingestion of a diet supplemented with 10% and 20% of coconut oil, which is rich in saturated fatty acids, on oxidative stress and cells stress signaling pathways.
After exposure to the diet for seven days, cellular and mitochondrial viability, lipid peroxidation and antioxidant enzymes SOD and CAT activity, and mRNA expression of antioxidant enzymes HSP83 and MPK2 were analyzed.
To confirm the damage effect of diet on flies, survival and lifespan were investigated.
The results revealed that the HFD augmented the rate of lipid peroxidation and SOD and CAT activity and induced a higher expression of HSP83 and MPK2 mRNA.
In parallel, levels of enzymes involved in lipid metabolism (ACSL1 and ACeCS1) were increased.
Our data demonstrate that association among metabolic changes, oxidative stress, and protein signalization might be involved in shortening the lifespan of flies fed with a HFD.
American Psychological Association (APA)
Trindade de Paula, Mariane& Poetini Silva, Márcia Rósula& Machado Araujo, Stífani& Cardoso Bortolotto, Vandreza& Barreto Meichtry, Luana& Zemolin, A. P. P.…[et al.]. 2016. High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113896
Modern Language Association (MLA)
Trindade de Paula, Mariane…[et al.]. High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1113896
American Medical Association (AMA)
Trindade de Paula, Mariane& Poetini Silva, Márcia Rósula& Machado Araujo, Stífani& Cardoso Bortolotto, Vandreza& Barreto Meichtry, Luana& Zemolin, A. P. P.…[et al.]. High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113896
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113896