HIV-1 Tat Regulates Occludin and Aβ Transfer Receptor Expression in Brain Endothelial Cells via RhoROCK Signaling Pathway
Joint Authors
Huang, Wen
Chen, Yan-lan
Wu, Xiang-hong
Jiang, Wenlin
Ye, Biao
Zhou, Xiaoting
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
HIV-1 transactivator protein (Tat) has been shown to play an important role in HIV-associated neurocognitive disorders.
The aim of the present study was to evaluate the relationship between occludin and amyloid-beta (Aβ) transfer receptors in human cerebral microvascular endothelial cells (hCMEC/D3) in the context of HIV-1-related pathology.
The protein expressions of occludin, receptor for advanced glycation end products (RAGE), and low-density lipoprotein receptor-related protein 1 (LRP1) in hCMEC/D3 cells were examined using western blotting and immunofluorescent staining.
The mRNA levels of occludin, RAGE, and LRP1 were measured using quantitative real-time polymerase chain reaction.
HIV-1 Tat at 1 µg/mL and the Rho inhibitor hydroxyfasudil (HF) at 30 µmol/L, with 24 h exposure, had no significant effect on hCMEC/D3 cell viability.
Treatment with HIV-1 Tat protein decreased mRNA and protein levels of occludin and LRP1 and upregulated the expression of RAGE; however, these effects were attenuated by HF.
These data suggest that the Rho/ROCK signaling pathway is involved in HIV-1 Tat-mediated changes in occludin, RAGE, and LRP1 in hCMEC/D3 cells.
HF may have a beneficial influence by protecting the integrity of the blood-brain barrier and the expression of Aβ transfer receptors.
American Psychological Association (APA)
Chen, Yan-lan& Huang, Wen& Jiang, Wenlin& Wu, Xiang-hong& Ye, Biao& Zhou, Xiaoting. 2016. HIV-1 Tat Regulates Occludin and Aβ Transfer Receptor Expression in Brain Endothelial Cells via RhoROCK Signaling Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113908
Modern Language Association (MLA)
Chen, Yan-lan…[et al.]. HIV-1 Tat Regulates Occludin and Aβ Transfer Receptor Expression in Brain Endothelial Cells via RhoROCK Signaling Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1113908
American Medical Association (AMA)
Chen, Yan-lan& Huang, Wen& Jiang, Wenlin& Wu, Xiang-hong& Ye, Biao& Zhou, Xiaoting. HIV-1 Tat Regulates Occludin and Aβ Transfer Receptor Expression in Brain Endothelial Cells via RhoROCK Signaling Pathway. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113908