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Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway
Joint Authors
Li, Min
Chen, Weiwei
Yu, Haitao
Yang, Yan
Xu, Xin-rong
Hang, Li
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-31
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The current study was aimed at evaluating the therapeutic implication of apigenin and to elucidate the underlying mechanism.
The tert-butyl hydroperoxide (t-BHP) at 200 μM was used to induce oxidative stress-associated injury in ARPE-19 cells.
Apigenin at concentrations less than 800 μM did not cause cytotoxic effects on ARPE-19 cells.
Cell viability assay showed that apigenin at 200 μM significantly promoted cell survival in t-BHP-treated ARPE-19 cells.
Additionally, apigenin at 100 μM significantly protected ARPE-19 cells from t-BHP-induced apoptosis.
Molecular examinations demonstrated that apigenin at 400 μM significantly upregulated the mRNA and protein expression of Nrf2 and stimulated its nuclear translocation in ARPE-19 cells treated with or without t-BHP.
Apigenin 400 μM also significantly elevated the expression of HO-1, NQO1, and GCLM at both mRNA and protein levels in the presence or absence of t-BHP.
Furthermore, apigenin at 400 μM significantly increased the activities of SOD, CAT, GSH-PX, and T-AOC and reduced the levels of ROS and MDA in t-BHP-treated ARPE-19 cells.
However, these effects of apigenin were all abolished by being transfected with Nrf2 siRNA.
Collectively, our current data indicated that apigenin exerted potent antioxidant properties in ARPE-19 cells challenged with t-BHP, which were dependent on activation of Nrf2 signaling.
American Psychological Association (APA)
Xu, Xin-rong& Li, Min& Chen, Weiwei& Yu, Haitao& Yang, Yan& Hang, Li. 2016. Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113922
Modern Language Association (MLA)
Xu, Xin-rong…[et al.]. Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1113922
American Medical Association (AMA)
Xu, Xin-rong& Li, Min& Chen, Weiwei& Yu, Haitao& Yang, Yan& Hang, Li. Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1113922
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113922