S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via AktNrf2HO-1 Pathway
Joint Authors
Zhu, Yizhun
Hou, Cui-Lan
Ma, Fen-Fen
Zhu, Yi-Chun
Li, Wenwen
Zhang, Laiyin
Huang, Yong
Li, Xinghui
Zhang, Aijie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-22
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
This study investigated the antioxidative effect of S-propargyl-cysteine (SPRC) on nonalcoholic fatty liver (NAFLD) by treating mice fed a methionine and choline deficient (MCD) diet with SPRC for four weeks.
We found that SPRC significantly reduced hepatic reactive oxygen species (ROS) and methane dicarboxylic aldehyde (MDA) levels.
Moreover, SPRC also increased the superoxide dismutase (SOD) activity.
By Western blot, we found that this protective effect of SPRC was importantly attributed to the regulated hepatic antioxidant-related proteins, including protein kinase B (Akt), heme oxygenase-1 (HO-1), nuclear factor erythroid 2-related factor 2 (Nrf2), and cystathionine γ-lyase (CSE, an enzyme that synthesizes hydrogen sulfide).
Next, we examined the detailed molecular mechanism of the SPRC protective effect using oleic acid- (OA-) induced HepG2 cells.
The results showed that SPRC significantly decreased intracellular ROS and MDA levels in OA-induced HepG2 cells by upregulating the phosphorylation of Akt, the expression of HO-1 and CSE, and the translocation of Nrf2.
SPRC-induced HO-1 expression and Nrf2 translocation were abolished by the phosphoinositide 3-kinase (PI3K) inhibitor LY294002.
Moreover, the antioxidative effect of SPRC was abolished by CSE inhibitor DL-propargylglycine (PAG) and HO-1 siRNA.
Therefore, these results proved that SPRC produced an antioxidative effect on NAFLD through the PI3K/Akt/Nrf2/HO-1 signaling pathway.
American Psychological Association (APA)
Li, Wenwen& Ma, Fen-Fen& Zhang, Laiyin& Huang, Yong& Li, Xinghui& Zhang, Aijie…[et al.]. 2016. S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via AktNrf2HO-1 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1113938
Modern Language Association (MLA)
Li, Wenwen…[et al.]. S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via AktNrf2HO-1 Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1113938
American Medical Association (AMA)
Li, Wenwen& Ma, Fen-Fen& Zhang, Laiyin& Huang, Yong& Li, Xinghui& Zhang, Aijie…[et al.]. S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via AktNrf2HO-1 Pathway. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1113938
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113938