Juglanthraquinone C Induces Intracellular ROS Increase and Apoptosis by Activating the AktFoxo Signal Pathway in HCC Cells
Joint Authors
Bao, Yong-li
Song, Zhen-bo
Hou, Ya-Qin
Yao, Yao
Yang, Cheng
Gao, Xiu-Li
Zhang, Wen-Jing
Sun, Lu-Guo
Yu, Chun-Lei
Huang, Yan-Xin
Wang, Guan-Nan
Li, Yu-Xin
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-22
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Juglanthraquinone C (JC), a naturally occurring anthraquinone extracted from Juglans mandshurica, could induce apoptosis of cancer cells.
This study aims to investigate the detailed cytotoxicity mechanism of JC in HepG2 and BEL-7402 cells.
The Affymetrix HG-U133 Plus 2.0 arrays were first used to analyze the mRNA expression exposed to JC or DMSO in HepG2 cells.
Consistent with the previous results, the data indicated that JC could induce apoptosis and hyperactivated Akt.
The Western blot analysis further revealed that Akt, a well-known survival protein, was strongly activated in HepG2 and BEL-7402 cells.
Furthermore, an obvious inhibitory effect on JC-induced apoptosis was observed when the Akt levels were decreased, while the overexpression of constitutively active mutant Akt greatly accelerated JC-induced apoptosis.
The subsequent results suggested that JC treatment suppressed nuclear localization and increased phosphorylated levels of Foxo3a, and the overexpression of Foxo3a abrogated JC-induced apoptosis.
Most importantly, the inactivation of Foxo3a induced by JC further led to an increase of intracellular ROS levels by suppressing ROS scavenging enzymes, and the antioxidant N-acetyl-L-cysteine and catalase successfully decreased JC-induced apoptosis.
Collectively, this study demonstrated that JC induced the apoptosis of hepatocellular carcinoma (HCC) cells by activating Akt/Foxo signaling pathway and increasing intracellular ROS levels.
American Psychological Association (APA)
Hou, Ya-Qin& Yao, Yao& Bao, Yong-li& Song, Zhen-bo& Yang, Cheng& Gao, Xiu-Li…[et al.]. 2015. Juglanthraquinone C Induces Intracellular ROS Increase and Apoptosis by Activating the AktFoxo Signal Pathway in HCC Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1113973
Modern Language Association (MLA)
Hou, Ya-Qin…[et al.]. Juglanthraquinone C Induces Intracellular ROS Increase and Apoptosis by Activating the AktFoxo Signal Pathway in HCC Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-18.
https://search.emarefa.net/detail/BIM-1113973
American Medical Association (AMA)
Hou, Ya-Qin& Yao, Yao& Bao, Yong-li& Song, Zhen-bo& Yang, Cheng& Gao, Xiu-Li…[et al.]. Juglanthraquinone C Induces Intracellular ROS Increase and Apoptosis by Activating the AktFoxo Signal Pathway in HCC Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1113973
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113973