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Endothelial Microparticle-Derived Reactive Oxygen Species: Role in Endothelial Signaling and Vascular Function
Joint Authors
Touyz, Rhian M.
Burger, Dylan
Turner, Maddison
Munkonda, Mercedes N.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-23
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Endothelial microparticles are effectors of endothelial damage; however mechanisms involved are unclear.
We examined the effects of eMPs on cultured endothelial cells (ECs) and isolated vessels and investigated the role of eMP-derived reactive oxygen species (ROS) and redox signaling in these processes.
eMPs were isolated from EC media and their ability to directly produce ROS was assessed by lucigenin and liquid chromatography.
Nicotinamide adenine dinucleotide phosphate oxidase (Nox) subunits were probed by Western blot.
ECs were treated with eMPs and effects on kinase signaling, superoxide anion ( O 2 ∙ - ) generation, and nitric oxide (NO) production were examined.
Acetylcholine-mediated vasorelaxation was assessed by myography in eMP-treated mesenteric arteries.
eMPs contained Nox1, Nox2, Nox4, p4 7 p h o x , p6 7 p h o x , and p2 2 p h o x and they produced ROS which was inhibited by the Nox inhibitor, apocynin.
eMPs increased phosphorylation of ERK1/2 and Src, increased O 2 ∙ - production, and decreased A23187-induced NO production in ECs.
Pretreatment of eMPs with apocynin diminished eMP-mediated effects on ROS and NO production but had no effect on eMP-mediated kinase activation or impairment in vasorelaxation.
Our findings identify a novel mechanism whereby eMP-derived ROS contributes to MP bioactivity.
These interactions may be important in conditions associated with vascular injury and increased eMP formation.
American Psychological Association (APA)
Burger, Dylan& Turner, Maddison& Munkonda, Mercedes N.& Touyz, Rhian M.. 2016. Endothelial Microparticle-Derived Reactive Oxygen Species: Role in Endothelial Signaling and Vascular Function. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113980
Modern Language Association (MLA)
Burger, Dylan…[et al.]. Endothelial Microparticle-Derived Reactive Oxygen Species: Role in Endothelial Signaling and Vascular Function. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1113980
American Medical Association (AMA)
Burger, Dylan& Turner, Maddison& Munkonda, Mercedes N.& Touyz, Rhian M.. Endothelial Microparticle-Derived Reactive Oxygen Species: Role in Endothelial Signaling and Vascular Function. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113980
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113980