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Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species
Joint Authors
Wilhelm, Jiří
Vytášek, Richard
Uhlík, Jiří
Vajner, Luděk
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-13
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Oxidative stress after birth led us to localize reactive oxygen and nitrogen species (RONS) production in the developing rat brain.
Brains were assessed a day prenatally and on postnatal days 1, 2, 4, 8, 14, 30, and 60.
Oxidation of dihydroethidium detected superoxide; 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate revealed hydrogen peroxide; immunohistochemical proof of nitrotyrosine and carboxyethyllysine detected peroxynitrite formation and lipid peroxidation, respectively.
Blue autofluorescence detected protein oxidation.
The foetuses showed moderate RONS production, which changed cyclically during further development.
The periods and sites of peak production of individual RONS differed, suggesting independent generation.
On day 1, neuronal/glial RONS production decreased indicating that increased oxygen concentration after birth did not cause oxidative stress.
Dramatic changes in the amount and the sites of RONS production occurred on day 4.
Nitrotyrosine detection reached its maximum.
Day 14 represented other vast alterations in RONS generation.
Superoxide production in arachnoidal membrane reached its peak.
From this day on, the internal elastic laminae of blood vessels revealed the blue autofluorescence.
The adult animals produced moderate levels of superoxide; all other markers reached their minimum.
There was a strong correlation between detection of nitrotyrosine and carboxyethyllysine probably caused by lipid peroxidation initiated with RONS.
American Psychological Association (APA)
Wilhelm, Jiří& Vytášek, Richard& Uhlík, Jiří& Vajner, Luděk. 2016. Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113982
Modern Language Association (MLA)
Wilhelm, Jiří…[et al.]. Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1113982
American Medical Association (AMA)
Wilhelm, Jiří& Vytášek, Richard& Uhlík, Jiří& Vajner, Luděk. Oxidative Stress in the Developing Rat Brain due to Production of Reactive Oxygen and Nitrogen Species. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1113982
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113982