Low-Dose Methylmercury-Induced Apoptosis and Mitochondrial DNA Mutation in Human Embryonic Neural Progenitor Cells
Joint Authors
Wu, Qing
Wang, Xinjin
Yan, Mengling
Zhao, Lina
Wu, Chunhua
Chang, Xiuli
Zhou, Zhijun
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-21
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Methylmercury (MeHg) is a long-lasting organic pollutant primarily found in the aquatic environment.
The developing brain is particularly sensitive to MeHg due to reduced proliferation of neural stem cell.
Although several mechanisms of MeHg-induced apoptosis have been defined in culture models, it remains unclear whether mitochondrial DNA (mtDNA) mutation is involved in the toxic effect of MeHg, especially in the neural progenitor cells.
In the present study, the ReNcell CX cell, a human neural progenitor cells (hNPCs) line, was exposed to nanomolar concentrations of MeHg (≤50 nM).
We found that MeHg altered mitochondrial metabolic function and induced apoptosis.
In addition, we observed that MeHg induced ROS production in a dose-dependent manner in hNPCs cells, which was associated with significantly increased expressions of ND1, Cytb, and ATP6.
To elucidate the mechanism underlying MeHg toxicity on mitochondrial function, we examined the ATP content and mitochondrial membrane potential in MeHg-treated hNPCs.
Our study showed that MeHg exposure led to decreased ATP content and reduced mitochondrial membrane potential, which failed to match the expansion in mtDNA copy number, suggesting impaired mtDNA.
Collectively, these results demonstrated that MeHg induced toxicity in hNPCs through altering mitochondrial function and inducing oxidative damage to mtDNA.
American Psychological Association (APA)
Wang, Xinjin& Yan, Mengling& Zhao, Lina& Wu, Qing& Wu, Chunhua& Chang, Xiuli…[et al.]. 2016. Low-Dose Methylmercury-Induced Apoptosis and Mitochondrial DNA Mutation in Human Embryonic Neural Progenitor Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113986
Modern Language Association (MLA)
Wang, Xinjin…[et al.]. Low-Dose Methylmercury-Induced Apoptosis and Mitochondrial DNA Mutation in Human Embryonic Neural Progenitor Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1113986
American Medical Association (AMA)
Wang, Xinjin& Yan, Mengling& Zhao, Lina& Wu, Qing& Wu, Chunhua& Chang, Xiuli…[et al.]. Low-Dose Methylmercury-Induced Apoptosis and Mitochondrial DNA Mutation in Human Embryonic Neural Progenitor Cells. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1113986
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1113986