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The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns
Joint Authors
Cejkova, Jitka
Cejka, Cestmir
Holan, Vladimir
Trosan, Peter
Zajicova, Alena
Javorkova, Eliska
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-05
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
The aim of this study was to examine whether mesenchymal stem cells (MSCs) and/or corneal limbal epithelial stem cells (LSCs) influence restoration of an antioxidant protective mechanism in the corneal epithelium and renewal of corneal optical properties changed after alkali burns.
The injured rabbit corneas (with 0.25 N NaOH) were untreated or treated with nanofiber scaffolds free of stem cells, with nanofiber scaffolds seeded with bone marrow MSCs (BM-MSCs), with adipose tissue MSCs (Ad-MSCs), or with LSCs.
On day 15 following the injury, after BM-MSCs or LSCs nanofiber treatment (less after Ad-MSCs treatment) the expression of antioxidant enzymes was restored in the regenerated corneal epithelium and the expressions of matrix metalloproteinase 9 (MMP9), inducible nitric oxide synthase (iNOS), α-smooth muscle actin (α-SMA), transforming growth factor-β1 (TGF-β1), and vascular endothelial factor (VEGF) were low.
The central corneal thickness (taken as an index of corneal hydration) increased after the injury and returned to levels before the injury.
In injured untreated corneas the epithelium was absent and numerous cells revealed the expressions of iNOS, MMP9, α-SMA, TGF-β1, and VEGF.
In conclusion, stem cell treatment accelerated regeneration of the corneal epithelium, restored the antioxidant protective mechanism, and renewed corneal optical properties.
American Psychological Association (APA)
Cejka, Cestmir& Holan, Vladimir& Trosan, Peter& Zajicova, Alena& Javorkova, Eliska& Cejkova, Jitka. 2016. The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114057
Modern Language Association (MLA)
Cejka, Cestmir…[et al.]. The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1114057
American Medical Association (AMA)
Cejka, Cestmir& Holan, Vladimir& Trosan, Peter& Zajicova, Alena& Javorkova, Eliska& Cejkova, Jitka. The Favorable Effect of Mesenchymal Stem Cell Treatment on the Antioxidant Protective Mechanism in the Corneal Epithelium and Renewal of Corneal Optical Properties Changed after Alkali Burns. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114057
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114057