CCL21 Facilitates Chemoresistance and Cancer Stem Cell-Like Properties of Colorectal Cancer Cells through AKTGSK-3βSnail Signals
Joint Authors
Lu, Lin-Lin
Chen, Xiao-Hui
Liu, Zong-Cai
Wu, Nong
Wang, Hao
Qi, Yi-Fei
Wang, Hong-Sheng
Cai, Shao Hui
Du, Jun
Zhang, Ge
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-28
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Some evidence indicated that chemoresistance associates with the acquisition of cancer stem-like properties.
Recent studies suggested that chemokines can promote the chemoresistance and stem cell properties in various cancer cells, while the underling mechanism is still not completely illustrated.
In our study, we found that CCL21 can upregulate the expression of P-glycoprotein (P-gp) and stem cell property markers such as Bmi-1, Nanog, and OCT-4 in colorectal cancer (CRC) HCT116 cells and then improve the cell survival rate and mammosphere formation.
Our results suggested that Snail was crucial for CCL21-mediated chemoresistance and cancer stem cell property in CRC cells.
Further, we observed that CCL21 treatment increased the protein but not mRNA levels of Snail, which suggested that CCL21 upregulates Snail via posttranscriptional ways.
The downstream signals AKT/GSK-3β mediated CCL21 induced the upregulation of Snail due to the fact that CCL21 treatment can obviously phosphorylate both AKT and GSK-3β.
The inhibitor of PI3K/Akt, LY294002 significantly abolished CCL21 induced chemoresistance and mammosphere formation of HCT116 cells.
Collectively, our results in the present study revealed that CCL21 can facilitate chemoresistance and stem cell property of CRC cells via the upregulation of P-gp, Bmi-1, Nanog, and OCT-4 through AKT/GSK-3β/Snail signals, which suggested a potential therapeutic approach to CRC patients.
American Psychological Association (APA)
Lu, Lin-Lin& Chen, Xiao-Hui& Zhang, Ge& Liu, Zong-Cai& Wu, Nong& Wang, Hao…[et al.]. 2015. CCL21 Facilitates Chemoresistance and Cancer Stem Cell-Like Properties of Colorectal Cancer Cells through AKTGSK-3βSnail Signals. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114064
Modern Language Association (MLA)
Lu, Lin-Lin…[et al.]. CCL21 Facilitates Chemoresistance and Cancer Stem Cell-Like Properties of Colorectal Cancer Cells through AKTGSK-3βSnail Signals. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1114064
American Medical Association (AMA)
Lu, Lin-Lin& Chen, Xiao-Hui& Zhang, Ge& Liu, Zong-Cai& Wu, Nong& Wang, Hao…[et al.]. CCL21 Facilitates Chemoresistance and Cancer Stem Cell-Like Properties of Colorectal Cancer Cells through AKTGSK-3βSnail Signals. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114064
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114064