Mitochondrial Alterations in Peripheral Mononuclear Blood Cells from Alzheimer’s Disease and Mild Cognitive Impairment Patients
Joint Authors
Delbarba, A.
Abate, G.
Prandelli, C.
Marziano, M.
Buizza, L.
Arce Varas, N.
Novelli, A.
Cuetos, F.
Martinez, C.
Lanni, C.
Memo, M.
Uberti, D.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
It is well recognized that mitochondrial dysfunction contributes to neurodegeneration occurring in Alzheimer’s disease (AD).
However, evidences of mitochondrial defects in AD peripheral cells are still inconclusive.
Here, some mitochondrial-encoded and nuclear-encoded proteins, involved in maintaining the correct mitochondria machine, were investigated in terms of protein expression and enzymatic activity in peripheral blood mononuclear cells (PBMCs) isolated from AD and Mild Cognitive Impairment (MCI) patients and healthy subjects.
In addition mitochondrial DNA copy number was measured by real time PCR.
We found some differences and some similarities between AD and MCI patients when compared with healthy subjects.
For example, cytochrome C and cytochrome B were decreased in AD, while MCI showed only a statistical reduction of cytochrome C.
On the other hand, both AD and MCI blood cells exhibited highly nitrated MnSOD, index of a prooxidant environment inside the mitochondria.
TFAM, a regulator of mitochondrial genome replication and transcription, was decreased in both AD and MCI patients’ blood cells.
Moreover also the mitochondrial DNA amount was reduced in PBMCs from both patient groups.
In conclusion these data confirmed peripheral mitochondria impairment in AD and demonstrated that TFAM and mtDNA amount reduction could be two features of early events occurring in AD pathogenesis.
American Psychological Association (APA)
Delbarba, A.& Abate, G.& Prandelli, C.& Marziano, M.& Buizza, L.& Arce Varas, N.…[et al.]. 2016. Mitochondrial Alterations in Peripheral Mononuclear Blood Cells from Alzheimer’s Disease and Mild Cognitive Impairment Patients. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1114068
Modern Language Association (MLA)
Delbarba, A.…[et al.]. Mitochondrial Alterations in Peripheral Mononuclear Blood Cells from Alzheimer’s Disease and Mild Cognitive Impairment Patients. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1114068
American Medical Association (AMA)
Delbarba, A.& Abate, G.& Prandelli, C.& Marziano, M.& Buizza, L.& Arce Varas, N.…[et al.]. Mitochondrial Alterations in Peripheral Mononuclear Blood Cells from Alzheimer’s Disease and Mild Cognitive Impairment Patients. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1114068
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114068