A Mechanistic Study on the Amiodarone-Induced Pulmonary Toxicity
Joint Authors
Al-Shammari, Bader
Khalifa, Mohamed
Yasser, Moustafa
Bakheet, Saleh A.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Amiodarone- (AM-) induced pulmonary toxicity (AIPT) is still a matter of research and is poorly understood.
In attempting to resolve this issue, we treated Sprague-Dawley rats with AM doses of 80 mg/kg/day/i.p.
for one, two, three, and four weeks.
The rats were weighed at days 7, 14, 21, and 28 and bronchoalveolar lavages (BAL) were obtained to determine total leukocyte count (TLC).
For each group, lung weighing, histopathology, and homogenization were performed.
Fresh homogenates were used for determination of ATP content, lipid peroxides, GSH, catalase, SOD, GPx, GR activities, NO, and hydroxyproline levels.
The results showed a significant decrease in body weight and GSH depletion together with an increase in both lung weight and lung/body weight coefficient in the first week.
Considerable increases in lung hydroxyproline level with some histopathological alterations were apparent.
Treatment for two weeks produced a significant increase in BAL fluid, TLC, GR activity, and NO level in lung homogenate.
The loss of cellular ATP and inhibition of most antioxidative protective enzymatic system appeared along with alteration in SOD activity following daily treatment for three weeks, while, in rats treated with AM for four weeks, more severe toxicity was apparent.
Histopathological diagnosis was mostly granulomatous inflammation and interstitial pneumonitis in rats treated for three and four weeks, respectively.
As shown, it is obvious that slow oedema formation is the only initiating factor of AIPT; all other mechanisms may occur as a consequence.
American Psychological Association (APA)
Al-Shammari, Bader& Khalifa, Mohamed& Bakheet, Saleh A.& Yasser, Moustafa. 2016. A Mechanistic Study on the Amiodarone-Induced Pulmonary Toxicity. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114096
Modern Language Association (MLA)
Al-Shammari, Bader…[et al.]. A Mechanistic Study on the Amiodarone-Induced Pulmonary Toxicity. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114096
American Medical Association (AMA)
Al-Shammari, Bader& Khalifa, Mohamed& Bakheet, Saleh A.& Yasser, Moustafa. A Mechanistic Study on the Amiodarone-Induced Pulmonary Toxicity. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114096
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114096