Oxidative Stress Assessment in Response to Ultraendurance Exercise: Thiols Redox Status and ROS Production according to Duration of a Competitive Race
Joint Authors
Montorsi, Michela
Moretti, Sarah
Mrakic-Sposta, Simona
Tonini, Annamaria
Accinni, Roberto
Vezzoli, Alessandra
Pratali, Lorenza
Dellanoce, Cinzia
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-18
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Purpose.
Response to an ultraendurance competitive race on thiols redox status, reactive oxygen species (ROS) production, and oxidative stress (OxS) was investigated according to duration.
Methods.
Twenty-four elite runners were examined: six completed 50 km and eighteen 100 km.
Blood and urine samples were collected before and immediately after the race.
Erythrocytes and plasma aminothiols by high-performance liquid chromatography, total antioxidant capacity (TAC), and OxS biomarkers (protein carbonyl (PC), thiobarbituric acid-reactive substances (TBARS), 8-isoprostane (8-iso-PGF2α), and 8-OH-2-deoxyguanosine (8-OH-dG)) by immunoenzymatic assays and ROS production by Electron Paramagnetic Resonance were assessed.
Results.
Significant increases ( P between <0.05 and <0.0001) were recorded in plasma total and oxidized aminothiols concentration and TAC ( P < 0.0001 ) only after 100 km: plasmatic (ROS production (+12 versus +29%), PC (+54 versus +115%), and TBARS (+28 versus +55%)) and urinary (8-OH-dG.creatinine−1 (+71 versus +158%) and 8-iso-PGF2α.creatinine−1 (+43 versus +135%)) concentrations for 50 and 100 km (duration 4 h 3′ versus 8 h 42′), respectively.
Conclusion.
Very prolonged ultraendurance exercise causes an increase in ROS production and OxS depending on specific biomarker examined but always linearly and directly related to exercise duration.
Redox status of erythrocytes was preserved.
A relationship between running performance and both prerace ROS production and antioxidant-redox status was found in 100 km race.
American Psychological Association (APA)
Vezzoli, Alessandra& Dellanoce, Cinzia& Mrakic-Sposta, Simona& Montorsi, Michela& Moretti, Sarah& Tonini, Annamaria…[et al.]. 2016. Oxidative Stress Assessment in Response to Ultraendurance Exercise: Thiols Redox Status and ROS Production according to Duration of a Competitive Race. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114114
Modern Language Association (MLA)
Vezzoli, Alessandra…[et al.]. Oxidative Stress Assessment in Response to Ultraendurance Exercise: Thiols Redox Status and ROS Production according to Duration of a Competitive Race. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1114114
American Medical Association (AMA)
Vezzoli, Alessandra& Dellanoce, Cinzia& Mrakic-Sposta, Simona& Montorsi, Michela& Moretti, Sarah& Tonini, Annamaria…[et al.]. Oxidative Stress Assessment in Response to Ultraendurance Exercise: Thiols Redox Status and ROS Production according to Duration of a Competitive Race. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114114
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114114