Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells
Joint Authors
Cen, Juan
Zhang, Li
Liu, Fangfang
Zhang, Feng
Ji, Bian-Sheng
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-15, 15 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-12
Country of Publication
Egypt
No. of Pages
15
Main Subjects
Abstract EN
Reactive oxygen species (ROS) play an important role in multidrug resistance (MDR).
This study aimed to investigate the effects of long-term ROS alteration on MDR in MCF-7 cells and to explore its underlying mechanism.
Our study showed both long-term treatments of H2O2 and glutathione (GSH) led to MDR with suppressed iROS levels in MCF-7 cells.
Moreover, the MDR cells induced by 0.1 μM H2O2 treatment for 20 weeks (MCF-7/ROS cells) had a higher viability and proliferative ability than the control MCF-7 cells.
MCF-7/ROS cells also showed higher activity or content of intracellular antioxidants like glutathione peroxidase (GPx), GSH, superoxide dismutase (SOD), and catalase (CAT).
Importantly, MCF-7/ROS cells were characterized by overexpression of MDR-related protein 1 (MRP1) and P-glycoprotein (P-gp), as well as their regulators NF-E2-related factor 2 (Nrf2), hypoxia-inducible factor 1 (HIF-1α), and the activation of PI3K/Akt pathway in upstream.
Moreover, several typical MDR mediators, including glutathione S-transferase-π (GST-π) and c-Myc and Protein Kinase Cα (PKCα), were also found to be upregulated in MCF-7/ROS cells.
Collectively, our results suggest that ROS may be critical in the generation of MDR, which may provide new insights into understanding of mechanisms of MDR.
American Psychological Association (APA)
Cen, Juan& Zhang, Li& Liu, Fangfang& Zhang, Feng& Ji, Bian-Sheng. 2016. Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1114168
Modern Language Association (MLA)
Cen, Juan…[et al.]. Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1114168
American Medical Association (AMA)
Cen, Juan& Zhang, Li& Liu, Fangfang& Zhang, Feng& Ji, Bian-Sheng. Long-Term Alteration of Reactive Oxygen Species Led to Multidrug Resistance in MCF-7 Cells. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1114168
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114168