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Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3KAkt and MAPKErk Activity in Mouse Embryonic Stem Cells
Joint Authors
Vasicek, Ondrej
Štefková, Kateřina
Kučera, Jan
Binó, Lucia
Jaroš, Josef
Večeřa, Josef
Pacherník, J.
Kubala, Lukas
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-14
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Reactive oxygen species (ROS) are important regulators of cellular functions.
In embryonic stem cells, ROS are suggested to influence differentiation status.
Regulated ROS formation is catalyzed primarily by NADPH-dependent oxidases (NOXs).
Apocynin and diphenyleneiodonium are frequently used inhibitors of NOXs; however, both exhibit uncharacterized effects not related to NOXs inhibition.
Interestingly, in our model of mouse embryonic stem cells we demonstrate low expression of NOXs.
Therefore we aimed to clarify potential side effects of these drugs.
Both apocynin and diphenyleneiodonium impaired proliferation of cells.
Surprisingly, we observed prooxidant activity of these drugs determined by hydroethidine.
Further, we revealed that apocynin inhibits PI3K/Akt pathway with its downstream transcriptional factor Nanog.
Opposite to this, apocynin augmented activity of canonical Wnt signaling.
On the contrary, diphenyleneiodonium activated both PI3K/Akt and Erk signaling pathways without affecting Wnt.
Our data indicates limits and possible unexpected interactions of NOXs inhibitors with intracellular signaling pathways.
American Psychological Association (APA)
Kučera, Jan& Binó, Lucia& Štefková, Kateřina& Jaroš, Josef& Vasicek, Ondrej& Večeřa, Josef…[et al.]. 2015. Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3KAkt and MAPKErk Activity in Mouse Embryonic Stem Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114189
Modern Language Association (MLA)
Kučera, Jan…[et al.]. Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3KAkt and MAPKErk Activity in Mouse Embryonic Stem Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1114189
American Medical Association (AMA)
Kučera, Jan& Binó, Lucia& Štefková, Kateřina& Jaroš, Josef& Vasicek, Ondrej& Večeřa, Josef…[et al.]. Apocynin and Diphenyleneiodonium Induce Oxidative Stress and Modulate PI3KAkt and MAPKErk Activity in Mouse Embryonic Stem Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114189
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114189