CD38 Deficiency Protects the Heart from IschemiaReperfusion Injury through Activating SIRT1FOXOs-Mediated Antioxidative Stress Pathway
Joint Authors
Ji, Guangju
Fu, Mingui
Deng, Keyu
Xin, Hongbo
Guan, Xiao-Hui
Liu, Xiao-Hong
Hong, Xuan
Zhao, Ning
Xiao, Yun-Fei
Wang, Ling-Fang
Tang, Ling
Jiang, Kai
Qian, Yi-Song
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-31
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Ischemia/reperfusion (I/R) injury induces irreversible oxidative stress damage to the cardiac muscle.
We previously observed that CD38 deficiency remarkably protects mouse embryonic fibroblasts (MEFs) from oxidative stress-induced injury.
However, whether CD38 deficiency protects from I/R injury in the heart is not explored.
Here, we showed that the hearts of CD38 deficient mice or wild type mice supplied with exogenous NAD were significantly protected from ischemia/reperfusion injury, seen as reduction of the myocardial infarct sizes when the mice were subjected to 30 min ischemia followed by 24 hours of reperfusion.
Consistently, the protection of CD38 deficiency on hypoxia/reoxygenation (H/R) injury was confirmed with a CD38 knockdown H9c2 stable cell line.
Furthermore, we observed that knockdown of CD38 remarkably inhibited ROS generation and intracellular Ca2+ overloading induced by H/R in H9c2 cells.
The FOXO1 and FOXO3 expressions were significantly elevated by H/R injury in CD38 knockdown cells compared with normal H9c2 cells.
The cell immunofluorescence assay showed that FOXO1 nuclear translocation was significantly increased in CD38 knockdown H9c2 cells.
In addition, we demonstrated that the increase of FOXO1 nuclear translocation was associated with the increased expressions of antioxidant catalase and SOD2 and the attenuated expression of the ROS generation enzyme NOX4.
In conclusion, our results provide new evidence that CD38 deficiency protects the heart from I/R injury through activating SIRT1/FOXOs-mediated antioxidative stress pathway.
American Psychological Association (APA)
Guan, Xiao-Hui& Liu, Xiao-Hong& Hong, Xuan& Zhao, Ning& Xiao, Yun-Fei& Wang, Ling-Fang…[et al.]. 2016. CD38 Deficiency Protects the Heart from IschemiaReperfusion Injury through Activating SIRT1FOXOs-Mediated Antioxidative Stress Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114190
Modern Language Association (MLA)
Guan, Xiao-Hui…[et al.]. CD38 Deficiency Protects the Heart from IschemiaReperfusion Injury through Activating SIRT1FOXOs-Mediated Antioxidative Stress Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1114190
American Medical Association (AMA)
Guan, Xiao-Hui& Liu, Xiao-Hong& Hong, Xuan& Zhao, Ning& Xiao, Yun-Fei& Wang, Ling-Fang…[et al.]. CD38 Deficiency Protects the Heart from IschemiaReperfusion Injury through Activating SIRT1FOXOs-Mediated Antioxidative Stress Pathway. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114190
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114190