Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROSp53p21Cip1Waf1 Pathway
Joint Authors
Zhang, Mingyu
Du, Yue
Lu, Renzhong
Shu, You
Zhao, Wei
Li, Zhuoyun
Zhang, Yu
Liu, Ruixue
Yang, Ti
Luo, Shenjian
Gao, Ming
Zhang, Yue
Zhang, Guiye
Liu, Jiaqi
Lu, Yanjie
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-15
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
In the present study, we demonstrated that bone marrow mesenchymal stem cells (BMSCs) of the 3rd passage displayed the senescence-associated phenotypes characterized with increased activity of SA-β-gal, altered autophagy, and increased G1 cell cycle arrest, ROS production, and expression of p53 and p 21 C i p 1 / W a f 1 compared with BMSCs of the 1st passage.
Cholesterol (CH) reduced the number of SA-β-gal positive cells in a dose-dependent manner in aging BMSCs induced by H2O2 and the 3rd passage BMSCs.
Moreover, CH inhibited the production of ROS and expression of p53 and p 21 C i p 1 / W a f 1 in both cellular senescence models and decreased the percentage of BMSCs in G1 cell cycle in the 3rd passage BMSCs.
CH prevented the increase in SA-β-gal positive cells induced by RITA (reactivation of p53 and induction of tumor cell apoptosis, a p53 activator) or 3-MA (3-methyladenine, an autophagy inhibitor).
Our results indicate that CH not only is a structural component of cell membrane but also functionally contributes to regulating cellular senescence by modulating cell cycle, autophagy, and the ROS/p53/ p 21 C i p 1 / W a f 1 signaling pathway.
American Psychological Association (APA)
Zhang, Mingyu& Du, Yue& Lu, Renzhong& Shu, You& Zhao, Wei& Li, Zhuoyun…[et al.]. 2016. Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROSp53p21Cip1Waf1 Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114206
Modern Language Association (MLA)
Zhang, Mingyu…[et al.]. Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROSp53p21Cip1Waf1 Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1114206
American Medical Association (AMA)
Zhang, Mingyu& Du, Yue& Lu, Renzhong& Shu, You& Zhao, Wei& Li, Zhuoyun…[et al.]. Cholesterol Retards Senescence in Bone Marrow Mesenchymal Stem Cells by Modulating Autophagy and ROSp53p21Cip1Waf1 Pathway. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1114206
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114206