Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1
Joint Authors
Zhang, Yanlin
Cao, Xueqin
Zhu, Wawa
Liu, Zhihua
Liu, Huihui
Zhou, Yande
Cao, Yongjun
Liu, Chunfeng
Xie, Ying
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-16
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Oxidized low-density lipoprotein- (Ox-LDL-) induced autophagy dysfunction in human vascular endothelial cells contributes to the development of atherosclerosis (AS).
Resveratrol (RSV) protects against Ox-LDL-induced endothelium injury.
The objective of this study was to determine the mechanisms underlying Ox-LDL-induced autophagy dysfunction and RSV-mediated protection in human umbilical vein endothelial cells (HUVECs).
The results showed that Ox-LDL suppressed the expression of sirtuin 1 (SIRT1) and increased LC3-II and sequestosome 1 (p62) protein levels without altering p62 mRNA levels in HUVECs.
Pretreatment with bafilomycin A1 (BafA1) to inhibit lysosomal degradation abrogated the Ox-LDL-induced increase in LC3-II protein level.
Ox-LDL increased colocalization of GFP and RFP puncta in mRFP-GFP-tandem fluorescent LC3- (tf-LC3-) transfected cells.
Moreover, Ox-LDL decreased the expression of mature cathepsin D and attenuated cathepsin D activity.
Pretreatment with RSV increased the expression of SIRT1 and LC3-II and increased p62 protein degradation.
RSV induced RFP-LC3 aggregation more than GFP-LC3 aggregation.
RSV restored lysosomal function and promoted Ox-LDL degradation in HUVECs.
All the protective effects of RSV were blocked after SIRT1 was knocked down.
These findings demonstrated that RSV upregulated the expression of SIRT1, restored lysosomal function, enhanced Ox-LDL-induced impaired autophagic flux, and promoted Ox-LDL degradation through the autophagy-lysosome degradation pathway in HUVECs.
American Psychological Association (APA)
Zhang, Yanlin& Cao, Xueqin& Zhu, Wawa& Liu, Zhihua& Liu, Huihui& Zhou, Yande…[et al.]. 2016. Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114226
Modern Language Association (MLA)
Zhang, Yanlin…[et al.]. Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1114226
American Medical Association (AMA)
Zhang, Yanlin& Cao, Xueqin& Zhu, Wawa& Liu, Zhihua& Liu, Huihui& Zhou, Yande…[et al.]. Resveratrol Enhances Autophagic Flux and Promotes Ox-LDL Degradation in HUVECs via Upregulation of SIRT1. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1114226
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114226