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Sulforaphane Protects Pancreatic Acinar Cell Injury by Modulating Nrf2-Mediated Oxidative Stress and NLRP3 Inflammatory Pathway
Joint Authors
Bhatia, Madhav
Dong, Zhaojun
Shang, Haixiao
Chen, Yong Q.
Pan, Li-Long
Sun, Jia
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-26
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Acute pancreatitis (AP) is characterized by early activation of intra-acinar proteases followed by acinar cell death and inflammation.
Cellular oxidative stress is a key mechanism underlying these pathological events.
Sulforaphane (SFN) is a natural organosulfur antioxidant with undescribed effects on AP.
Here we investigated modulatory effects of SFN on cellular oxidation and inflammation in AP.
AP was induced by cerulean hyperstimulation in BALB/c mice.
Treatment group received a single dose of 5 mg/kg SFN for 3 consecutive days before AP.
We found that SFN administration attenuated pancreatic injury as evidenced by serum amylase, pancreatic edema, and myeloperoxidase, as well as by histological examination.
SFN administration reverted AP-associated dysregulation of oxidative stress markers including pancreatic malondialdehyde and redox enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx).
In acinar cells, SFN treatment upregulated nuclear factor erythroid 2-related factor 2 (Nrf2) expression and Nrf2-regulated redox genes including quinoneoxidoreductase-1, heme oxidase-1, SOD1, and GPx1.
In addition, SFN selectively suppressed cerulein-induced activation of the nucleotide-binding domain leucine-rich repeat containing family, pyrin domain-containing 3 (NLRP3) inflammasome, in parallel with reduced nuclear factor- (NF-) κB activation and modulated NF-κB-responsive cytokine expression.
Together, our data suggested that SFN modulates Nrf2-mediated oxidative stress and NLRP3/NF-κB inflammatory pathways in acinar cells, thereby protecting against AP.
American Psychological Association (APA)
Dong, Zhaojun& Shang, Haixiao& Chen, Yong Q.& Pan, Li-Long& Bhatia, Madhav& Sun, Jia. 2016. Sulforaphane Protects Pancreatic Acinar Cell Injury by Modulating Nrf2-Mediated Oxidative Stress and NLRP3 Inflammatory Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114247
Modern Language Association (MLA)
Dong, Zhaojun…[et al.]. Sulforaphane Protects Pancreatic Acinar Cell Injury by Modulating Nrf2-Mediated Oxidative Stress and NLRP3 Inflammatory Pathway. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1114247
American Medical Association (AMA)
Dong, Zhaojun& Shang, Haixiao& Chen, Yong Q.& Pan, Li-Long& Bhatia, Madhav& Sun, Jia. Sulforaphane Protects Pancreatic Acinar Cell Injury by Modulating Nrf2-Mediated Oxidative Stress and NLRP3 Inflammatory Pathway. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114247
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114247