Cathepsin B Regulates Collagen Expression by Fibroblasts via Prolonging TLR2NF-κB Activation
Joint Authors
Yu, Weixian
Zhou, Yanmin
Meng, Jie
Li, Xue
Ni, Junjun
Liu, Yicong
Nakanishi, Hiroshi
Wu, Zhou
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-28
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Fibroblasts are essential for tissue repair due to producing collagens, and lysosomal proteinase cathepsin B (CatB) is involved in promoting chronic inflammation.
We herein report that CatB regulates the expression of collagens III and IV by fibroblasts in response to a TLR2 agonist, lipopolysaccharide from Porphyromonas gingivalis (P.g.
LPS).
In cultured human BJ fibroblasts, mRNA expression of CatB was significantly increased, while that of collagens III and IV was significantly decreased at 24 h after challenge with P.g.
LPS (1 μg/mL).
The P.g.
LPS-decreased collagen expression was completely inhibited by CA-074Me, the specific inhibitor of CatB.
Surprisingly, expression of collagens III and IV was significantly increased in the primary fibroblasts from CatB-deficient mice after challenge with P.g.
LPS.
The increase of CatB was accompanied with an increase of 8-hydroxy-2′-deoxyguanosine (8-OHdG) and a decrease of IκBα.
Furthermore, the P.g.
LPS-increased 8-OHdG and decreased IκBα were restored by CA-074Me.
Moreover, 87% of CatB and 86% of 8-OHdG were colocalized with gingival fibroblasts of chronic periodontitis patients.
The findings indicate the critical role of CatB in regulating the expression of collagens III and IV by fibroblasts via prolonging TLR2/NF-κB activation and oxidative stress.
CatB-specific inhibitors may therefore improve chronic inflammation-delayed tissue repair.
American Psychological Association (APA)
Li, Xue& Wu, Zhou& Ni, Junjun& Liu, Yicong& Meng, Jie& Yu, Weixian…[et al.]. 2016. Cathepsin B Regulates Collagen Expression by Fibroblasts via Prolonging TLR2NF-κB Activation. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114254
Modern Language Association (MLA)
Li, Xue…[et al.]. Cathepsin B Regulates Collagen Expression by Fibroblasts via Prolonging TLR2NF-κB Activation. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1114254
American Medical Association (AMA)
Li, Xue& Wu, Zhou& Ni, Junjun& Liu, Yicong& Meng, Jie& Yu, Weixian…[et al.]. Cathepsin B Regulates Collagen Expression by Fibroblasts via Prolonging TLR2NF-κB Activation. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114254