Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya
Joint Authors
Abigael, Ouko
Sheila, Okoth
Joutsjoki, Vesa
Amugune, Nelson Onzere
Source
Issue
Vol. 2018, Issue 2018 (31 Dec. 2018), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2018-11-18
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In this study, the possibility of sexual reproduction in sampled Aspergillus flavus strains was evaluated by assessing the distribution of mating type (MAT) genes, which are known to control sexual character among fungi, for two counties in Kenya.
Forty-four isolates from Nandi and Makueni counties were genotyped by MAT using a multiplex polymerase chain reaction assay.
The primer pair for the MAT1-1 amplified a 396 base pair (bp) fragment containing an α-box motif, and MAT1-2 primers targeted a 270 bp segment with a high mobility group protein.
The MAT1-2 genes dominated in both regions although the frequency was higher in Nandi (75%) than in Makueni (54.17%).
There were no MAT1-1 genes sampled in Nandi, and in Makueni their proportion was 15.91%.
The percentage of isolates that amplified for both MAT genes in Makueni was 9.09%, while in Nandi it was 11.36%.
Currently, use of aggressive aflatoxin non-producing A.
flavus strains as biocontrol is the most promising preharvest aflatoxin control strategy in Kenya.
However, we address the possibility of introduced biocontrol strains to breed with existing aflatoxin producing strains in nature, which could lead to the generation of A.
flavus offspring capable of aflatoxin production while also being aggressive colonizers and possibly increasing the burden of aflatoxin exposure in food.
American Psychological Association (APA)
Abigael, Ouko& Sheila, Okoth& Amugune, Nelson Onzere& Joutsjoki, Vesa. 2018. Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya. Advances in Agriculture،Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1114320
Modern Language Association (MLA)
Abigael, Ouko…[et al.]. Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya. Advances in Agriculture No. 2018 (2018), pp.1-6.
https://search.emarefa.net/detail/BIM-1114320
American Medical Association (AMA)
Abigael, Ouko& Sheila, Okoth& Amugune, Nelson Onzere& Joutsjoki, Vesa. Characterization of Mating Type Genes in Aspergillus flavus Populations from Two Locations in Kenya. Advances in Agriculture. 2018. Vol. 2018, no. 2018, pp.1-6.
https://search.emarefa.net/detail/BIM-1114320
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114320