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Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats
Joint Authors
Gong, Xuezhong
Duan, Yiru
Zheng, Junli
Wang, Yiquan
Wang, Guohua
Norgren, Svante
Hei, Tom K.
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-26
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Contrast-induced nephropathy (CIN) is a leading cause of hospital-acquired acute kidney injury (AKI) due to apoptosis induced in renal tubular cells.
Our previous study demonstrated the novel N-acetylcysteine amide (NACA); the amide form of N-acetyl cysteine (NAC) prevented renal tubular cells from contrast-induced apoptosis through inhibiting p38 MAPK pathway in vitro.
In the present study, we aimed to compare the efficacies of NACA and NAC in preventing CIN in a well-established rat model and investigate whether thioredoxin-1 (Trx1) and apoptosis signal-regulating kinase 1 (ASK1) act as the potential activator for p38 MAPK.
NACA significantly attenuated elevations of serum creatinine, blood urea nitrogen, and biomarkers of AKI.
At equimolar concentration, NACA was more effective than NAC in reducing histological changes of renal tubular injuries.
NACA attenuated activation of p38 MAPK signal, reduced oxidative stress, and diminished apoptosis.
Furthermore, we demonstrated that contrast exposure resulted in Trx1 downregulation and increased ASK1/p38 MAPK phosphorylation, which could be reversed by NACA and NAC.
To our knowledge, this is the first report that Trx1 and ASK1 are involved in CIN.
Our study highlights a renal protective role of NACA against CIN through modulating Trx1 and ASK1/p38 MAPK pathway to result in the inhibition of apoptosis among renal cells.
American Psychological Association (APA)
Gong, Xuezhong& Duan, Yiru& Zheng, Junli& Wang, Yiquan& Wang, Guohua& Norgren, Svante…[et al.]. 2016. Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1114450
Modern Language Association (MLA)
Gong, Xuezhong…[et al.]. Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-11.
https://search.emarefa.net/detail/BIM-1114450
American Medical Association (AMA)
Gong, Xuezhong& Duan, Yiru& Zheng, Junli& Wang, Yiquan& Wang, Guohua& Norgren, Svante…[et al.]. Nephroprotective Effects of N-Acetylcysteine Amide against Contrast-Induced Nephropathy through Upregulating Thioredoxin-1, Inhibiting ASK1p38MAPK Pathway, and Suppressing Oxidative Stress and Apoptosis in Rats. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1114450
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114450