Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes
Joint Authors
Souza, Verónica
Bucio, Leticia
Miranda, Roxana U.
Gomez-Quiroz, Luis Enrique
Gutiérrez-Ruiz, María Concepción
López-Islas, Anayelly
Pérez-Aguilar, Benjamin
Palestino-Domínguez, Mayrel
Chagoya de Sánchez, Victoria
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-12-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Obesity and alcohol consumption are risk factors for hepatic steatosis, and both commonly coexist.
Our objective was to evaluate the effect of ethanol and acetaldehyde on primary hepatocytes obtained from mice fed for two days with a high cholesterol (HC) diet.
HC hepatocytes increased lipid and cholesterol content.
HC diet sensitized hepatocytes to the toxic effect of ethanol and acetaldehyde.
Cyp2E1 content increased with HC diet, as well as in those treated with ethanol or acetaldehyde, while the activity of this enzyme determined in microsomes increased in the HC and in all ethanol treated hepatocytes, HC and CW.
Oxidized proteins were increased in the HC cultures treated or not with the toxins.
Transmission electron microscopy showed endoplasmic reticulum (ER) stress and megamitochondria in hepatocytes treated with ethanol as in HC and the ethanol HC treated hepatocytes.
ER stress determined by PERK content was increased in ethanol treated hepatocytes from HC mice and CW.
Nuclear translocation of ATF6 was observed in HC hepatocytes treated with ethanol, results that indicate that lipids overload and ethanol treatment favor ER stress.
Oxidative stress, ER stress, and mitochondrial damage underlie potential mechanisms for increased damage in steatotic hepatocyte treated with ethanol.
American Psychological Association (APA)
López-Islas, Anayelly& Chagoya de Sánchez, Victoria& Pérez-Aguilar, Benjamin& Palestino-Domínguez, Mayrel& Souza, Verónica& Miranda, Roxana U.…[et al.]. 2015. Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1114725
Modern Language Association (MLA)
López-Islas, Anayelly…[et al.]. Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1114725
American Medical Association (AMA)
López-Islas, Anayelly& Chagoya de Sánchez, Victoria& Pérez-Aguilar, Benjamin& Palestino-Domínguez, Mayrel& Souza, Verónica& Miranda, Roxana U.…[et al.]. Cholesterol Enhances the Toxic Effect of Ethanol and Acetaldehyde in Primary Mouse Hepatocytes. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1114725
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114725