Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner
Joint Authors
Yao, Weifeng
Li, Haobo
Han, Xue
Liu, Zipeng
Zhang, Zhong-jun
Xia, Zhengyuan
Hei, Ziqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-07
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Oxidative stress plays a critical role in the pathogenesis of intestinal ischemia reperfusion (IIR) injury.
Enhancement in endogenous Lipoxin A4 (LXA4), a potent antioxidant and mediator, is associated with attenuation of IIR.
However, the effects of LXA4 on IIR injury and the potential mechanisms are unknown.
In a rat IIR (ischemia 45 minutes and subsequent reperfusion 6 hours) model, IIR caused intestinal injury, evidenced by increased serum diamine oxidase, D-lactic acid, intestinal-type fatty acid-binding protein, and the oxidative stress marker 15-F2t-Isoprostane.
LXA4 treatment significantly attenuated IIR injury by reducing mucosal 15-F2t-Isoprostane and elevating endogenous antioxidant superoxide dismutase activity, accompanied with Keap1/Nrf2 pathway activation.
Meanwhile, LXA4 receptor antagonist Boc-2 reversed the protective effects of LXA4 on intestinal injury but failed to affect the oxidative stress and the related Nrf2 pathway.
Furthermore, Nrf2 antagonist brusatol reversed the antioxidant effects conferred by LXA4 and led to exacerbation of intestinal epithelium cells oxidative stress and apoptosis, finally resulting in a decrease of survival rate of rat.
Meanwhile, LXA4 pretreatment upregulated nuclear Nrf2 level and reduced hypoxia/reoxygenation-induced IEC-6 cell damage and Nrf2 siRNA reversed this protective effect of LXA4 in vitro.
In conclusion, these findings suggest that LXA4 ameliorates IIR injury by activating Keap1/Nrf2 pathway in a LXA4 receptor independent manner.
American Psychological Association (APA)
Han, Xue& Yao, Weifeng& Liu, Zipeng& Li, Haobo& Zhang, Zhong-jun& Hei, Ziqing…[et al.]. 2016. Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114745
Modern Language Association (MLA)
Han, Xue…[et al.]. Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1114745
American Medical Association (AMA)
Han, Xue& Yao, Weifeng& Liu, Zipeng& Li, Haobo& Zhang, Zhong-jun& Hei, Ziqing…[et al.]. Lipoxin A4 Preconditioning Attenuates Intestinal Ischemia Reperfusion Injury through Keap1Nrf2 Pathway in a Lipoxin A4 Receptor Independent Manner. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1114745
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114745