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In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet
Joint Authors
Freitas, Isabel
Ferrigno, Andrea
Gruppi, Cristian
Vaccarone, Rita
Tira, M. Enrica
Bertone, Vittorio
Milanesi, Gloria
Tarantola, Eleonora
Boncompagni, Eleonora
Vairetti, Mariapia
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-01-06
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
Nonalcoholic fatty liver disease (NAFLD) is a serious health problem in developed countries.
We documented the effects of feeding with a NAFLD-inducing, methionine- and choline-deficient (MCD) diet, for 1–4 weeks on rat liver oxidative stress, with respect to a control diet.
Glycogen, neutral lipids, ROS, peroxidated proteins, and SOD2 were investigated using histochemical procedures; ATP, GSH, and TBARS concentrations were investigated by biochemical dosages, and SOD2 expression was investigated by Western Blotting.
In the 4-week-diet period, glycogen stores decreased whereas lipid droplets, ROS, and peroxidated proteins expression (especially around lipid droplets of hepatocytes) increased.
SOD2 immunostaining decreased in poorly steatotic hepatocytes but increased in the thin cytoplasm of macrosteatotic cells; a trend towards a quantitative decrease of SOD expression in homogenates occurred after 3 weeks.
ATP and GSH values were significantly lower for rats fed with the MCD diet with respect to the controls.
An increase of TBARS in the last period of the diet is in keeping with the high ROS production and low antioxidant defense; these TBARS may promote protein peroxidation around lipid droplets.
Since these proteins play key roles in lipid mobilization, storage, and metabolism, this last information appears significant, as it points towards a previously misconsidered target of NAFLD-associated oxidative stress that might be responsible for lipid dysfunction.
American Psychological Association (APA)
Freitas, Isabel& Boncompagni, Eleonora& Tarantola, Eleonora& Gruppi, Cristian& Bertone, Vittorio& Ferrigno, Andrea…[et al.]. 2016. In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114747
Modern Language Association (MLA)
Freitas, Isabel…[et al.]. In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-14.
https://search.emarefa.net/detail/BIM-1114747
American Medical Association (AMA)
Freitas, Isabel& Boncompagni, Eleonora& Tarantola, Eleonora& Gruppi, Cristian& Bertone, Vittorio& Ferrigno, Andrea…[et al.]. In Situ Evaluation of Oxidative Stress in Rat Fatty Liver Induced by a Methionine- and Choline-Deficient Diet. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-14.
https://search.emarefa.net/detail/BIM-1114747
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114747