Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin
Joint Authors
von Woedtke, Thomas
Bekeschus, Sander
Schmidt, Anke
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-17, 17 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-04
Country of Publication
Egypt
No. of Pages
17
Main Subjects
Abstract EN
Oxidative stress illustrates an imbalance between radical formation and removal.
Frequent redox stress is critically involved in many human pathologies including cancer, psoriasis, and chronic wounds.
However, reactive species pursue a dual role being involved in signaling on the one hand and oxidative damage on the other.
Using a HaCaT keratinocyte cell culture model, we investigated redox regulation and inflammation to periodic, low-dose oxidative stress after two, six, eight, ten, and twelve weeks.
Chronic redox stress was generated by recurrent incubation with cold physical plasma-treated cell culture medium.
Using transcriptome microarray technology, we identified both acute ROS-stress responses as well as numerous adaptions after several weeks of redox challenge.
We determined a differential expression (2-fold, FDR < 0.01, p<0.05) of 260 genes that function in inflammation and redox homeostasis, such as cytokines (e.g., IL-6, IL-8, and IL-10), growth factors (e.g., CSF2, FGF, and IGF-2), and antioxidant enzymes (e.g., HMOX, NQO1, GPX, and PRDX).
Apoptotic signaling was affected rather modestly, especially in p53 downstream targets (e.g., BCL2, BBC3, and GADD45).
Strikingly, the cell-protective heat shock protein HSP27 was strongly upregulated (p<0.001).
These results suggested cellular adaptions to frequent redox stress and may help to better understand the inflammatory responses in redox-related diseases.
American Psychological Association (APA)
Schmidt, Anke& von Woedtke, Thomas& Bekeschus, Sander. 2016. Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1114845
Modern Language Association (MLA)
Schmidt, Anke…[et al.]. Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-17.
https://search.emarefa.net/detail/BIM-1114845
American Medical Association (AMA)
Schmidt, Anke& von Woedtke, Thomas& Bekeschus, Sander. Periodic Exposure of Keratinocytes to Cold Physical Plasma: An In Vitro Model for Redox-Related Diseases of the Skin. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-17.
https://search.emarefa.net/detail/BIM-1114845
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114845