Sulforaphane Attenuates Contrast-Induced Nephropathy in Rats via Nrf2HO-1 Pathway

Abstract EN

Background.

Oxidative stress plays an important role in the pathogenesis of contrast-induced nephropathy (CIN).

The aim of this study was to investigate the antioxidant effects of sulforaphane (SFN) in a rat model of CIN and a cell model of oxidative stress in HK2 cells.

Methods.

Rats were randomized into four groups ( n = 6 per group): control group, Ioversol group (Ioversol-induced CIN), Ioversol + SFN group (CIN rats pretreated with SFN), and SFN group (rats treated with SFN).

Renal function tests, malondialdehyde (MDA), and reactive oxygen species (ROS) were measured.

Western blot, real-time polymerase chain reaction analysis, and immunohistochemical analysis were performed for nuclear factor erythroid-derived 2-like 2 (Nrf2) and heme oxygenase-1 (HO-1) detection.

Results.

Serum blood urea nitrogen (BUN), creatinine, and renal tissue MDA were increased after contrast exposure.

Serum BUN, creatinine, and renal tissue MDA were decreased in the Ioversol + SFN group as compared with those in the Ioversol group.

SFN increased the expression of Nrf2 and HO-1 in CIN rats and in Ioversol-induced injury HK2 cells.

SFN increased cell viability and attenuated ROS level in vitro.

Conclusions.

SFN attenuates experimental CIN in vitro and in vivo.

This effect is suggested to activate the Nrf2 antioxidant defenses pathway.