Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3KAkt Signaling Pathways in Mouse Neuro-2a Cells
Joint Authors
Pan, Xiaoqi
Zhao, Wanyun
Li, Tao
Zhang, Changchun
Shi, Nian
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-18, 18 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-04-07
Country of Publication
Egypt
No. of Pages
18
Main Subjects
Abstract EN
Trimethyltin chloride (TMT) is a classic neurotoxicant that can cause severe neurodegenerative diseases.
Some signaling pathways involving cell death play pivotal roles in the central nervous system.
In this study, the role of Sonic Hedgehog (Shh) and PI3K/Akt pathways in TMT-induced apoptosis and protective effect of Lycium barbarum polysaccharides (LBP) on mouse neuro-2a (N2a) cells were investigated.
Results showed that TMT treatment significantly enhanced apoptosis, upregulated proapoptotic Bax, downregulated antiapoptotic Bcl-2 expression, and increased caspase-3 activity in a dose-dependent manner in N2a cells.
TMT induced oxidative stress in cells, performing reactive oxygen species (ROS) and malondialdehyde (MDA) excessive generation, and superoxide dismutase (SOD) activity reduction.
TMT significantly decreased phosphorylated glycogen synthase kinase-3β (GSK-3β) and inhibited Shh and PI3K/Akt pathways.
However, the addition of LBP upregulated GSK-3β phosphorylation, activated Shh and PI3K/Akt pathways, and eventually reduced apoptosis and oxidative stress caused by TMT.
The interaction between Shh and PI3K/Akt pathways was clarified by specific PI3K inhibitor LY294002 or Shh inhibitor GDC-0449.
Moreover, LY294002 and GDC-0449 pretreatment both induced phosphorylated GSK-3β downregulation and significantly promoted apoptosis induced by TMT.
These results suggest that LBP could reduce TMT-induced N2a cells apoptosis by regulating GSK-3β phosphorylation, Shh, and PI3K/Akt signaling pathways.
American Psychological Association (APA)
Zhao, Wanyun& Pan, Xiaoqi& Li, Tao& Zhang, Changchun& Shi, Nian. 2016. Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3KAkt Signaling Pathways in Mouse Neuro-2a Cells. Oxidative Medicine and Cellular Longevity،Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1114850
Modern Language Association (MLA)
Zhao, Wanyun…[et al.]. Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3KAkt Signaling Pathways in Mouse Neuro-2a Cells. Oxidative Medicine and Cellular Longevity No. 2016 (2016), pp.1-18.
https://search.emarefa.net/detail/BIM-1114850
American Medical Association (AMA)
Zhao, Wanyun& Pan, Xiaoqi& Li, Tao& Zhang, Changchun& Shi, Nian. Lycium barbarum Polysaccharides Protect against Trimethyltin Chloride-Induced Apoptosis via Sonic Hedgehog and PI3KAkt Signaling Pathways in Mouse Neuro-2a Cells. Oxidative Medicine and Cellular Longevity. 2016. Vol. 2016, no. 2016, pp.1-18.
https://search.emarefa.net/detail/BIM-1114850
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1114850