A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue
Joint Authors
Bonina, Francesco
Lo Furno, Debora
Graziano, Adriana Carol Eleonora
Avola, Rosanna
Giuffrida, Rosario
Perciavalle, Vincenzo
Mannino, Giuliana
Cardile, Venera
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-15
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The aim of this research was to assess the impact of a well-characterized extract from Citrus bergamia juice on adipogenesis and/or lipolysis using mesenchymal stem cells from human adipose tissue as a cell model.
To evaluate the effects on adipogenesis, some cell cultures were treated with adipogenic medium plus 10 or 100 μg/mL of extract.
To determine the properties on lipolysis, additional mesenchymal stem cells were cultured with adipogenic medium for 14 days and after this time added with Citrus bergamia for further 14 days.
To verify adipogenic differentiation, oil red O staining at 7, 14, 21, and 28 days was performed.
Moreover, the expression of peroxisome proliferator-activated receptor gamma (PPAR-γ), adipocytes fatty acid-binding protein (A-FABP), adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), monoglyceride lipase (MGL), 5′-adenosine monophosphate-activated protein kinase (AMPK)α1/2, and pAMPKα1/2 was evaluated by Western blot analysis and the release of glycerol by colorimetric assay.
Citrus bergamia extract suppressed the accumulation of intracellular lipids in mesenchymal stem cells during adipogenic differentiation and promoted lipolysis by repressing the expression of adipogenic genes and activating lipolytic genes.
Citrus bergamia extract could be a useful natural product for improving adipose mobilization in obesity-related disorders.
American Psychological Association (APA)
Lo Furno, Debora& Graziano, Adriana Carol Eleonora& Avola, Rosanna& Giuffrida, Rosario& Perciavalle, Vincenzo& Bonina, Francesco…[et al.]. 2016. A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue. PPAR Research،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1115319
Modern Language Association (MLA)
Lo Furno, Debora…[et al.]. A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue. PPAR Research No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1115319
American Medical Association (AMA)
Lo Furno, Debora& Graziano, Adriana Carol Eleonora& Avola, Rosanna& Giuffrida, Rosario& Perciavalle, Vincenzo& Bonina, Francesco…[et al.]. A Citrus bergamia Extract Decreases Adipogenesis and Increases Lipolysis by Modulating PPAR Levels in Mesenchymal Stem Cells from Human Adipose Tissue. PPAR Research. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1115319
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1115319