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IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells
Joint Authors
Thornton, Shantaé M.
Krolopp, James E.
Abbott, Marcia J.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-12, 12 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-21
Country of Publication
Egypt
No. of Pages
12
Main Subjects
Abstract EN
Molecular mediators of metabolic processes, to increase energy expenditure, have become a focus for therapies of obesity.
The discovery of cytokines secreted from the skeletal muscle (SKM), termed “myokines,” has garnered attention due to their positive effects on metabolic processes.
Interleukin-15 (IL-15) is a myokine that has numerous positive metabolic effects and is linked to the PPAR family of mitochondrial regulators.
Here, we aimed to determine the importance of PPARα and/or PPARδ as targets of IL-15 signaling.
C2C12 SKM cells were differentiated for 6 days and treated every other day with IL-15 (100 ng/mL), a PPARα inhibitor (GW-6471), a PPARδ inhibitor (GSK-3787), or both IL-15 and the inhibitors.
IL-15 increased mitochondrial activity and induced PPARα, PPARδ, PGC1α, PGC1β, UCP2, and Nrf1 expression.
There was no effect of inhibiting PPARα, in combination with IL-15, on the aforementioned mRNA levels except for PGC1β and Nrf1.
However, with PPARδ inhibition, IL-15 failed to induce the expression levels of PGC1α, PGC1β, UCP2, and Nrf1.
Further, inhibition of PPARδ abolished IL-15 induced increases in citrate synthase activity, ATP production, and overall mitochondrial activity.
IL-15 had no effects on mitochondrial biogenesis.
Our data indicates that PPARδ activity is required for the beneficial metabolic effects of IL-15 signaling in SKM.
American Psychological Association (APA)
Thornton, Shantaé M.& Krolopp, James E.& Abbott, Marcia J.. 2016. IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells. PPAR Research،Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1115325
Modern Language Association (MLA)
Thornton, Shantaé M.…[et al.]. IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells. PPAR Research No. 2016 (2016), pp.1-12.
https://search.emarefa.net/detail/BIM-1115325
American Medical Association (AMA)
Thornton, Shantaé M.& Krolopp, James E.& Abbott, Marcia J.. IL-15 Mediates Mitochondrial Activity through a PPARδ-Dependent-PPARα-Independent Mechanism in Skeletal Muscle Cells. PPAR Research. 2016. Vol. 2016, no. 2016, pp.1-12.
https://search.emarefa.net/detail/BIM-1115325
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1115325