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Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ
Joint Authors
Aji, Kaisaier
Maimaijiang, Munila
Aimaiti, Abudusaimi
Rexiati, Mulati
Azhati, Baihetiya
Tusong, Hamulati
Cui, Lei
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-14
Country of Publication
Egypt
No. of Pages
9
Abstract EN
The multifunctional Ca2+/calmodulin-dependent protein kinase II (CaMKII) is known to participate in maintenance and switches of smooth muscle cell (SMC) phenotypes.
However, which isoform of CaMKII is involved in differentiation of adult mesenchymal stem cells into contractile SMCs remains unclear.
In the present study, we detected γ isoform of CaMKII in differentiation of human adipose derived stem cells (hASCs) into SMCs that resulted from treatment with TGF-β1 and BMP4 in combination for 7 days.
The results showed that CaMKIIγ increased gradually during differentiation of hASCs as determined by real-time PCR and western blot analysis.
The siRNA-mediated knockdown of CaMKIIγ decreased the protein levels and transcriptional levels of smooth muscle contractile markers (a-SMA, SM22a, calponin, and SM-MHC), while CaMKIIγ overexpression increases the transcriptional and protein levels of smooth muscle contractile markers.
These results suggested that γ isoform of CaMKII plays a significant role in smooth muscle differentiation of hASCs.
American Psychological Association (APA)
Aji, Kaisaier& Maimaijiang, Munila& Aimaiti, Abudusaimi& Rexiati, Mulati& Azhati, Baihetiya& Tusong, Hamulati…[et al.]. 2016. Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1115987
Modern Language Association (MLA)
Aji, Kaisaier…[et al.]. Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1115987
American Medical Association (AMA)
Aji, Kaisaier& Maimaijiang, Munila& Aimaiti, Abudusaimi& Rexiati, Mulati& Azhati, Baihetiya& Tusong, Hamulati…[et al.]. Differentiation of Human Adipose Derived Stem Cells into Smooth Muscle Cells Is Modulated by CaMKIIγ. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1115987
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1115987