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Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells
Joint Authors
Zandieh-Doulabi, Behrouz
Bravenboer, Nathalie
Kleverlaan, Cornelis J.
Bastidas-Coral, Angela P.
Bakker, Astrid D.
Forouzanfar, Tim
Klein-Nulend, Jenneke
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-09-07
Country of Publication
Egypt
No. of Pages
9
Abstract EN
During the initial stages of bone repair, proinflammatory cytokines are released within the injury site, quickly followed by a shift to anti-inflammatory cytokines.
The effect of pro- and anti-inflammatory cytokines on osteogenic differentiation of mesenchymal stem cells is controversial.
Here, we investigated the effect of the proinflammatory cytokines TNF-α, IL-6, IL-8, and IL-17F and the anti-inflammatory cytokine IL-4 on proliferation and osteogenic differentiation of human adipose stem cells (hASCs).
hASCs were treated with TNF-α, IL-6, IL-8, IL-17F, or IL-4 (10 ng/mL) for 72 h mimicking bone repair.
TNF-α reduced collagen type I gene expression but increased hASC proliferation and ALP activity.
IL-6 also strongly enhanced ALP activity (18-fold), as well as bone nodule formation by hASCs.
IL-8 did not affect proliferation or osteogenic gene expression but reduced bone nodule formation.
IL-17F decreased hASC proliferation but enhanced ALP activity.
IL-4 enhanced osteocalcin gene expression and ALP activity but reduced RUNX2 gene expression and bone nodule formation.
In conclusion, all cytokines studied have both enhancing and reducing effects on osteogenic differentiation of hASCs, even when applied for 72 h only.
Some cytokines, specifically IL-6, may be suitable to induce osteogenic differentiation of mesenchymal stem cells as a strategy for enhancing bone repair.
American Psychological Association (APA)
Bastidas-Coral, Angela P.& Bakker, Astrid D.& Zandieh-Doulabi, Behrouz& Kleverlaan, Cornelis J.& Bravenboer, Nathalie& Forouzanfar, Tim…[et al.]. 2016. Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116001
Modern Language Association (MLA)
Bastidas-Coral, Angela P.…[et al.]. Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116001
American Medical Association (AMA)
Bastidas-Coral, Angela P.& Bakker, Astrid D.& Zandieh-Doulabi, Behrouz& Kleverlaan, Cornelis J.& Bravenboer, Nathalie& Forouzanfar, Tim…[et al.]. Cytokines TNF-α, IL-6, IL-17F, and IL-4 Differentially Affect Osteogenic Differentiation of Human Adipose Stem Cells. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116001
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116001