The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation
Joint Authors
Park, Sung-Hwan
Ju, Ji Hyeon
Kim, Youngkyun
Rim, Yeri Alice
Yi, Hyoju
Park, Narae
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-04
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Human induced pluripotent stem cells (hiPSCs) have demonstrated great potential for differentiation into diverse tissues.
We report a straightforward and highly efficient method for the generation of iPSCs from PBMCs.
By plating the cells serially to a newly coated plate by centrifugation, this protocol provides multiple healthy iPSC colonies even from a small number of PBMCs.
The generated iPSCs expressed pluripotent markers and differentiated into all three germ layer lineages.
The protocol can also be used with umbilical cord blood mononuclear cells (CBMCs).
In this study, we present a simple and efficient protocol that improved the yield of iPSCs from floating cells such as PBMCs and CBMCs by serial plating and centrifugation.
American Psychological Association (APA)
Kim, Youngkyun& Rim, Yeri Alice& Yi, Hyoju& Park, Narae& Park, Sung-Hwan& Ju, Ji Hyeon. 2016. The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116006
Modern Language Association (MLA)
Kim, Youngkyun…[et al.]. The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116006
American Medical Association (AMA)
Kim, Youngkyun& Rim, Yeri Alice& Yi, Hyoju& Park, Narae& Park, Sung-Hwan& Ju, Ji Hyeon. The Generation of Human Induced Pluripotent Stem Cells from Blood Cells: An Efficient Protocol Using Serial Plating of Reprogrammed Cells by Centrifugation. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116006
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116006