![](/images/graphics-bg.png)
Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells
Joint Authors
Zhang, Lei
Dang, Rui-Jie
Yang, Yan-Mei
Cui, Dian-Chao
Li, Ping
Ni, Yan-Li
Hao, Tong
Wang, Changyong
Jiang, Xiao-Xia
Fang, Nan-Zhu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-11-16
Country of Publication
Egypt
No. of Pages
11
Abstract EN
As stromal cells and recently confirmed mesenchymal stem cells, OP9 cells support hematopoiesis stem cell (HSC) differentiation into the B lymphocyte lineage, yet Delta-like-1 (DL1) overexpressing OP9 (OP9DL1) cells promote the development of early T lymphocytes from HSC.
However, the immunomodulatory capacity of OP9 or OP9DL1 on mature B and T cell proliferation has not been elucidated.
Here, we show that OP9 and OP9DL1 have similar proliferation capacities and immunophenotypes except DL1 expression.
Compared with OP9, OP9DL1 displayed more osteogenesis and less adipogenesis when cultured in the respective induction media.
Both OP9 and OP9DL1 inhibited mature B and T cell proliferation.
Furthermore, OP9 showed stronger inhibition on B cell proliferation and OP9DL1 exhibited stronger inhibition on T cell proliferation.
With stimulation, both OP9 and OP9DL1 showed increased nitrate oxide (NO) production.
The NO levels of OP9 were higher than that of OP9DL1 when stimulated with TNFα/IFNγ or LPS/IL4.
Taken together, our study reveals a previously unrecognized role of OP9 and OP9DL1 in mature B and T cell proliferation.
DL1 overexpression alone changed the properties of OP9 cells in addition to their role in early B cell development.
American Psychological Association (APA)
Zhang, Lei& Dang, Rui-Jie& Yang, Yan-Mei& Cui, Dian-Chao& Li, Ping& Ni, Yan-Li…[et al.]. 2015. Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells. Stem Cells International،Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116077
Modern Language Association (MLA)
Zhang, Lei…[et al.]. Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1116077
American Medical Association (AMA)
Zhang, Lei& Dang, Rui-Jie& Yang, Yan-Mei& Cui, Dian-Chao& Li, Ping& Ni, Yan-Li…[et al.]. Delta-Like-1 Changes the Immunomodulatory Property of OP9 Cells. Stem Cells International. 2015. Vol. 2016, no. 2016, pp.1-11.
https://search.emarefa.net/detail/BIM-1116077
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116077