In Vitro Osteogenic Potential of Green Fluorescent Protein Labelled Human Embryonic Stem Cell-Derived Osteoprogenitors
Joint Authors
Islam, Intekhab
Sriram, Gopu
Li, Mingming
Zou, Yu
Li, Lulu
Handral, Harish K.
Rosa, Vinicus
Cao, Tong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-28
Country of Publication
Egypt
No. of Pages
9
Abstract EN
Cellular therapy using stem cells in bone regeneration has gained increasing interest.
Various studies suggest the clinical utility of osteoprogenitors-like mesenchymal stem cells in bone regeneration.
However, limited availability of mesenchymal stem cells and conflicting evidence on their therapeutic efficacy limit their clinical application.
Human embryonic stem cells (hESCs) are potentially an unlimited source of healthy and functional osteoprogenitors (OPs) that could be utilized for bone regenerative applications.
However, limited ability to track hESC-derived progenies in vivo greatly hinders translational studies.
Hence, in this study, we aimed to establish hESC-derived OPs (hESC-OPs) expressing green fluorescent protein (GFP) and to investigate their osteogenic differentiation potential in vitro.
We fluorescently labelled H9-hESCs using a plasmid vector encoding GFP.
The GFP-expressing hESCs were differentiated into hESC-OPs.
The hESC-OPsGFP+ stably expressed high levels of GFP, CD73, CD90, and CD105.
They possessed osteogenic differentiation potential in vitro as demonstrated by increased expression of COL1A1, RUNX2, OSTERIX, and OPG transcripts and mineralized nodules positive for Alizarin Red and immunocytochemical expression of osteocalcin, alkaline phosphatase, and collagen-I.
In conclusion, we have demonstrated that fluorescently labelled hESC-OPs can maintain their GFP expression for the long term and their potential for osteogenic differentiation in vitro.
In future, these fluorescently labelled hESC-OPs could be used for noninvasive assessment of bone regeneration, safety, and therapeutic efficacy.
American Psychological Association (APA)
Islam, Intekhab& Sriram, Gopu& Li, Mingming& Zou, Yu& Li, Lulu& Handral, Harish K.…[et al.]. 2016. In Vitro Osteogenic Potential of Green Fluorescent Protein Labelled Human Embryonic Stem Cell-Derived Osteoprogenitors. Stem Cells International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116090
Modern Language Association (MLA)
Islam, Intekhab…[et al.]. In Vitro Osteogenic Potential of Green Fluorescent Protein Labelled Human Embryonic Stem Cell-Derived Osteoprogenitors. Stem Cells International Vol. 2016, no. 2016 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1116090
American Medical Association (AMA)
Islam, Intekhab& Sriram, Gopu& Li, Mingming& Zou, Yu& Li, Lulu& Handral, Harish K.…[et al.]. In Vitro Osteogenic Potential of Green Fluorescent Protein Labelled Human Embryonic Stem Cell-Derived Osteoprogenitors. Stem Cells International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1116090
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1116090